泡菜中优良乳酸菌的筛选及高效混合菌株发酵剂研制

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1、河北农业大学硕士学位论文泡菜中优良乳酸菌的筛选及高效混合菌株发酵剂研制姓名：吴蕊申请学位级别：硕士专业：微生物学指导教师：田洪涛20060611Study011FiltrationLacticAcidBacteriafromPicklingVegetableandHigh—activityAdmixtureBacterinmStrainStarterCultureAuther：Ⅵ，uRujSupervisor：Prof．HongtaoTianMajor：MicrobiologyAbstractPicklingvegetableisasortoftradition

2、aryfermentfood,ithassepecialSe刀tseandabundancenutrition，itispopularedforthepeople．Inthispaper,lacticacidbacteriawereisolatedandidentifiedfrommanysortsofpicklingvegetableforhavingexcellentcharacteristicsinacidproductionandtheabilityfordegradationthenitrate．11kfermentationcharacterisic

3、softhesestainswerealsostudied．Ithasisolatedstrainsandoptimizationcollocationforfinelacticacidbacteria,studiedtheprimaryfactorswhichaffectmanufacturingtechnologyofpicklingvegetable．Thecheapenrichmentmediumsuitedforindustrialprocessingandfreeze-dryingprotectantwel'escreened．Thetechnolo

4、gyofenrichmentcultureforlacticacidbacteria,ccUconcentration,lyophilizationwerestudied．Thefermentativeactivityofthefreeze-dryingstarterincabbagebrinewereexamined．86strainsoflacticacidbacteriawereisolatedandidentifiedfromeightpicklingvegetable．Fivestrainswereselectedforhavingexcellentc

5、haracteristicsinacidproductionandtheabilityfordegradationthenitrate．Formorphologyappraisementandthetestforphysiologyandbiochemicalevent,therearetwoLactobacillusplantarum，one，oneLactobacilluspentosus,oneLeuconostocmesenteroidessubsp．dextrainicum．Thefermentationcharacterisicsofthesefiv

6、esrainswerealsostudied．ItmakeLeuconostocmesenteroidessubsp．LN①，Lactobacillusplantarum儿②andLactobacillusbrevisLS②proratecombinationarrangeinpairsforstarter．ItscecnoutLN0)：JL∞1：2、LN①：LS◇l：2、LN①：几②：LS②=I：l：1atechoicenessstarter,andmakethethreestarterindifferenttemperaturefermentcabbie，m

7、akesureIO'Cisthebesttemperatureforthefermentca_bbage．Tomatojuicemedium,carrotjuicemediumandcabbagemediumwhichaleapttOgainandseparateareobtainedbycellgroethexperimented．TheresultshowedthatcabbagejuicewasthebestbasemediumforLactobacillusbrevisLS②．111eeffectofadding12kindsofdifferentnut

8、rientfactors