根癌农杆菌介导的ANTICTLA4HS83基因遗传转化甘薯的研究

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1、西南交通大学硕士学位论文根癌农杆菌介导的ANTI-CTLA-4-HS83基因遗传转化甘薯的研究姓名：许培芳申请学位级别：硕士专业：生物化学与分子生物学指导教师：李萍;阎文昭20080401西南交通大学硕士研究生学位论文第1l页AbstractPlant-basedexpressionofpharmaceuticalproteinsisacOSt—effectivealtern．ativetotraditionallarge—scaleproductionsystemssuchasmicrobialferment

2、ation，insectandmammaliancellcultures，andtransgenicanimals．Plantsarethemosteconomicalmakersofbiomassusingonlysunlight，water，carbondioxideandminerals．Theyofferapropermodificationsystemforeukaryoticproteins，suchasfolding，post-tr．anslationalmodification，andsub—c

3、ellulartargeting；moreover，theyavoidanimalcell—culturecontaminantssuchasmammalianpmhogensandextraneousviralorbacterialcomponents．Sincethehumangrowthhormonewasexpressedintransgenictobaccoin1986，manymorepharmaceuticallyrelevantproteinshavebeensuccessfullyproduc

4、edinplants．TheproteinsexpressedinplantsCanbeextractedfrom．wholeplantpartsororganellesandusedasasourceformedicinalapplications．Productionofsweetpotato(Ipomoeabatatas(L)Lava．)ranksfifthafterthatofrice，wheat，maizeandwhitepotato，amongthefoodcropsoftheworld．Sweet

5、potatoisoneofthemostimportantcropsfordevelopingcountries，whereitrankssecondaccordingtoitsproductionvalue，andfifthaccordingtoitscaloriecontri--butionforhumans．Sweetpotatohaveahighproductionyieldofbiomass，needlessnutrientsfromthesoilthanmostothercrops．Establis

6、hingsweetpotatoasaproductionsiteforthedeliveryofrecOmbinantproteinsisworthstrivingfor．Singlechainfragmentvariables(scFv)anti—CTLA-hs83isonekindoftargetfragmentsforimmunotoxins，itCallselectivelylinktheactivatedTcells，possessesthefeaturesofspecificity，highacii

7、vityandlowimmunogenicity．Soitmaybehavegoodprospectforpreventionandcureoftransplantrejection．西南交通大学硕士研究生学位论文第1II页Inthisstudy，wedescribeallagrobacteriumbinaryvectorcarryingsinglechainvariablefragment(scFv)anti··CqT．A·-hs83genewassuccessfullyconstructedbasedonp

8、CAMBIAl301，thentransformedsweetpotatoplantswiththisbinaryvectorusingA．tumefaciens-mediatedtransformation，30hygromycinresistantplantswereobtained．Hygromycinresistantplantsweretestedbypolymerasech