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1、小麦种质系山农030-1抗白粉病基因的分子标记定位(amplificationofspecificbands)andperformancetype(diseaseresistance)isinconsistent.Correlationanalysiswasmadetoexplainthecorrelationbetween1RS-specificmarkersandpowderymildewresistancegeneofShannong030-1,theresultsshowedtheyhadnocorrelation.Allindicatedthatthepowderymildew
2、resistanceofShannong030-1hadnothingtodowithrye1RSchromosome.3.FivespecificmolecularmarkerslinkedtothepowderymildewresistancegeneofShannong030-1wereselectedfrom2492G-SSR,EST-SSRandSTSmarkersviathePreferredSmallGroup(PSG)inShannong030-1/Yannong15F2.ThePm030-1wasmappedbythelinkageanalysisofasegregat
3、ingF2population.ThePm030-1waslinkedtofivespecificmarkers(CFE27,Xcfd81,Xcfd266,Xcfd18andXcfd25)andtheirmostlikelyorderwasCFE27–Xcfd81–Pm–Xcfd266–Xcfd18–Xcfd25at3.2,1.3,8.6,24.3and24.3cM,respect-tively.InShannong030-1/KavkazF2,thePm030-1waslinkedtotwospecificmarkers(CFE27andXcfd81)andtheirmostlikel
4、yorderwasCFE27–Pm–Xcfd81at3.7and0.9cM,respectively;InShannong030-1/ChancellorF2,thePm030-1waslinkedtotwospecificmarkers(Xcfd81andCFE27)andtheirmostlikelyorderwasCFE27–Xcfd81–Pmat11.3and3.0cM,respectively;InShannong030-1/HuixianhongF2,thePm030-1waslinkedtothreespecificmarkers(CFE27,Xcfd81andXcfd26
5、6)andtheirmostlikelyorderwasXcfd266–CFE27–Pm–Xcfd81at13.8,2.2and3.4cM,respectively.TheseresultsindicatedthatmarkersXcfd81andCFE27werethetightlylinkedmarkerstothePm030-1.4.ThethePm030-1waslocatedonchromosome5D,accordingtotheinfection-typeofE09Bgtisolateandmappingresults,andweconcludethatitmightbep
6、owderymildewresistancegenePm2oranewalleleofthePm2locus.Themolecularmarkersdevelopedinthisstudyareusefulformarker-assistedselection(MAS)andgenepyramidingofpowderymildewresistancegenesinwheatbreedingprograms.Keywords:Commonwheat;Powderymildew;Shannong030-1;Resistancegene;Molec-ularmarker4符号说明AFLP:A
7、mplifiedfragmentlengthpolymorphism,扩增片段长度多态性Bp:BasePair,碱基对BSA:BulkedSegregantAnalysis,分离群体分组分析法BSA:BovineSerumAlbumin,牛血清白蛋白DNA:Doxyribonucleicaicd,脱氧核糖核酸dNTP:DeoxyribonucleosideTriphosphate,脱氧核糖核苷三磷酸EB:EthidiumBromid
