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1、棉花学报CottonScience2015袁27渊6冤院561―569棉仁植酸含量的离子色谱测定方法研究陆智辉袁阎君袁辛文慧袁徐晓建袁祝水金袁陈进红*渊浙江大学农业与生物技术学院农学系/浙江省作物种质资源重点实验室袁杭州310058冤摘要院为准确测定棉仁植酸含量袁本研究从提取工艺尧色谱条件改进等方面入手袁优化建立了棉仁植酸含量的快速提取及离子色谱测定方法院棉仁粉样品烘干后用乙醚脱脂袁以10mL0.66mol窑L－1的HCl提取袁沸水浴1h袁4℃震荡12h袁离心分离袁取上清液稀释并纯化后上样分析曰采用DIONEXICS-2000离子色谱仪尧
2、AG11-HCGuard(4mm伊50mm)保护柱和AG11-HCAnalytical(4mm伊250mm)分离柱袁在30.0mmol窑L－1的KOH为淋洗液袁流动相流速为1.0mL窑min－1袁采样时间为25min袁抑制电流为75mA袁柱温30℃袁进样量20滋L的条件下进行检测遥结果表明袁棉仁植酸的回归曲线2为0.999袁在检测浓度内线性关系良好曰各项指标相对标准偏差均在5%以内袁平均回收率为98.64%～102.31%袁检出限渊样品中检出待测物的最小浓度冤为0.059滋g窑mL－1袁定量限渊样品中待测物能被定量测定的最低量冤为0.19
3、6滋g窑mL－1遥关键词院棉仁曰植酸曰离子色谱中图分类号院O657.7+5∶S562文献标志码院A文章编号院1002-7807渊2015冤06-0561-0910.11963/issn.1002-7807.201506008*LuZhihui,YanJun,XinWenhui,XuXiaojian,ZhuShuijin,ChenJinhong(310058,)Cottonseedmealisanimportantplantproteinresourceoffeedindustryandbreedingindustry,althoughit
4、containsmanyantinutritionalfactors.Phyticacid,asoneofthemainlyantinutritionalfactor,cannotbedigestedeasilybymonogastrican-imals,suchasfowlsandpigs,consequentlyresultsinthedecreasedbio-availabilityofmaterialelementsandleadstotheenvi-ronmentpollutionbythephytateinexcreta.Th
5、usfar,studyofbreedingcottonscontaininglowphyticacidgraduallybecomesthehotspot,andthemethodoffullyextractingphyticacidfromcottonseedsandprecisemeasurementisbecomingmoreandmoreimportantandnecessary.Anewmethodfortherapidextractionanddeterminationofphyticacidincottonseedkerne
6、lsbyionchromatographywasdevelopedinthisstudy.Afterthediethylethertreatmentofdrycottonseedkernelpowder,thesamplesweresuspendedin10ml0.66mol窑L－1HClandboiledfor1hour.Thenthesampleswereshakenat4℃for12hoursandcen-trifuged.Thesupernatantwasdilutedandpurifiedforloading.Toanalyze
7、thesample,DIONEXICS-2000ionchromatography,AG11-HCGuard(4mm伊50mm)protectioncolumnandAG11-HCGuard(4mm伊250mm)separationcolumnwereusedincontrolledconditions(washingbuffer:30.0mmol窑L－1KOH;reamspeed:1.0mL窑min－1;collectingtime:25min;75mAcur-rent;temperature:30℃;loadingamount:20滋
8、L).TheresultsshowedthatR2oflinearregressionis0.999,whichmeansthere-gressionrelationisgreat.Inadd
