资源描述：
《转Cry30Fa1基因抗褐飞虱水稻的获得及鉴定》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、中国水稻科学(ChinJRiceSci),２０１６,３０(３):２５６－２６４http://www．ricesci．cn２５６DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１９０转Cry３０Fa１基因抗褐飞虱水稻的获得及鉴定王海鹏黄晓西梁越洋朱军张翠霞王秀梅贡常委郑爱萍邓其明李双成王玲霞李平王世全∗(四川农业大学水稻研究所,四川温江６１１１３０;∗通讯联系人,EＧmail:sqwangscau＠１６３．com)DevelopmentandIdentificationofInsectResistantTransgenicRicewithCry３０
2、Fa１GeneWANGHaiＧpeng,HUANGXiaoＧxi,LIANGYueＧyang,ZHUJun,ZHANGCuiＧxia,WANGXiuＧmei,GONGChangＧwei,ZHENGAiＧping,DENGQiＧming,LIShuangＧcheng,WANGLingＧxia,LIPing,WANGShiＧquan∗(RiceResearchInstitute,SichuanAgriculturalUniversity,Wenjiang６１１１３０,China;∗Correspondingauthor,EＧmail:sqwangscau＠１６３c
3、．om)WANGHaipeng,HUANGXiaoxi,LIANGYueyang,etal．DevelopmentandidentificationofinsectresistanttransgenicricewithCry３０Fa１gene．ChinJRiceSci,２０１６,３０(３):２５６Ｇ２６４．Abstract:ModifiedCry３０Fa１,aBacillusthuringiensis(Bt)geneencodingahighＧefficiencyinsecticidalproteintothebrownplanthopper(BPH),was
4、introducedintoShuhui８１８(R８１８)byAgrobacteriumＧmediatedgenetictransformationand４６transgenelineswereobtained．WeconfirmedthevalidtransformationbyRTＧqRCRandWesternblotattranscriptionallevelandtranslationallevel,andfixedthestableexpressionCry３０Fa１inhomozygosis．Theclassicalpedigreebreeding
5、methodswerealsousedtoselecttheimportantagronomictraitsintransgeniclines．Furthermore,weevaluatedtheresistancetoBPHofdifferentlinesingrowthchamberandfield,andfoundthattheR８１８ＧCry３０FaldisplayedhigherresistancethanuntransformedR８１８inseedlingstage．WealsoobservedthereducedpopulationofBPHf
6、eedingthetransgeniclines,suggestingthatthetransformedCry３０Fa１enhancedresistibilitytoBPHinrice．WecultivatednewBTproteintransgenicricerestorerlineR８１８ＧCry３０Fal,whichmayprovidenewBPHresistancematerialtothreelinehybridricebreedingandenrichBPHresistancegermplasmresourcesinrice．Keywords:b
7、rownplanthopper;AgrobacteriumＧmediatedmethod;insectresistance;Cry３０Fa１王海鹏,黄晓西,梁越洋,等．转Cry３０Fa１基因抗褐飞虱水稻的获得及鉴定．中国水稻科学,２０１６,３０(３):２５６Ｇ２６４．摘要:将编码高效杀褐飞虱蛋白的苏云金芽胞杆菌基因Cry３０Fa１密码子改造后,通过农杆菌介导法转入蜀恢８１８(R８１８),并最终获得４６个转基因植株.通过定量PCR及WesternBlot鉴定了Cry３０Fa１在转录水平和蛋白水平的表达,并通过分子检测固定稳定表达的抗性基因,并结合传统育种系谱法选
8、择具有优良农艺性状株系.对选育的株系在
