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1、生物工程学报ChinJBiotech2008,October25;24(10):1824-1827journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2008InstituteofMicrobiology,CAS&CSM,Allrightsreserved研究报告发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略王杰鹏,谭天伟北京化工大学生命科学与技术学院,北京100029摘要:利用酿酒酵母菌株高密度发酵法生产S-腺苷蛋氨酸关
2、键的影响因素之一是前体L-蛋氨酸的补加策略。本研究采用一支经过常规诱变处理的S-腺苷蛋氨酸优势积累菌株酿酒酵母SAM0801,通过5L发酵罐高密度发酵实验研究,考察了6种补加策略,最终确定了L-蛋氨酸的加入时机为30h左右,当菌体干重达到100g/L时,补加量为每罐40gL-蛋氨酸,发酵58h左右达到最高生物量干重168g/L,产量14.48g/L。关键词:S-腺苷蛋氨酸,酿酒酵母,高密度发酵,L-蛋氨酸Pre-L-methionineFeedingStrategyforS-adenosyl-L-methio
3、nineFermentativeProductionJiepengWang,andTianweiTanCollegeofLifeScienceandTechnology,BeijingUniversityofChemicalTechnology,Beijing100029,ChinaAbstract:TheyieldofS-adenosyl-L-methionine(SAM)onhigh-cell-densityfermentationbysaccharomycescerevisiaeismostlyaffe
4、ctedbythefeedingstrategyofpre-L-methionine.ThemutantstrainSAM0801thatcouldaccumulatemoreSAMwasusedinthisstudy.Sixhigh-cell-densityfermentationexperimentsin5LfermentorwereinvestigatedtogettheoptimalfeedingtimeandamountofL-methionine.Theresultsshowedthatwhen4
5、0gL-methioninewasaddedinthefermentorafter30hfermentation,adrycellweightof100g/Lwasachieved.Underthiscondition,after58hfermentation,boththedrycellweightandtheyieldofSAMreachedthemaximum,168g/Land14.48g/Lrespectively.Keywords:S-adenosyl-L-methionine,Saccharom
6、ycescerevisiae,high-cell-densityfermentation,L-methionine[1]S-腺苷蛋氨酸(SAM)是生物体内重要的代谢时间内均没有文献将其超越。近年来,国内研究机构[4]中间物质,作为基团供体参与多种生化反应,临床在SAM发酵工艺上取得了重大突破:何俊云等报道[2][2][3]上用于肝病和抑郁症等疾病的治疗。Shiozaki了其利用重组毕赤酵母在5L发酵罐中发酵114h于1986年率先报道了利用酿酒酵母在10L发酵罐SAM产量达到13.5g/L(未给出前体的浓度和
7、利用[5]中发酵培养7dSAM产量达到10.8g/L,此后很长率);胡晓清等报道了利用重组毕赤酵母在15LReceived:March11,2008;Accepted:April25,2008Supportedby:theNationalNaturalScienceFoundationofChina(No.20576013),theHiTechResearchandDevelopmentProgram(“863”Program)ofChina(Nos.2006AA020102,2006AA020103and2
8、006AA020201),theStateKeyDevelopmentProgramforBasicResearch(“973”Program)ofChina(Nos.2007CB707804,2007CB714304),theNaturalScienceFoundationofBeijing,China(No.2071002),theScienceandTechnologyProgramofBei
