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1、JournalofExperimentalBotany,Vol.58,No.1,pp.103–112,2007IntracellularCompartmentation:BiogenesisandFunctionSpecialIssuedoi:10.1093/jxb/erj209AdvanceAccesspublication27June,2006SPECIALISSUEPAPERTheproteomicsofplantcellmembranesSetsukoKomatsu*,HirosatoKonishiandMa
2、kotoHashimotoNationalInstituteofAgrobiologicalSciences,2-1-2Kannondai,Tsukuba305-8602,JapanReceived11December2005;Accepted21March2006AbstractInplantcells,aswellasinanimalcells,theplasmamem-branecontrolsmanyprimarycellularfunctions,suchasMembraneproteinsareinvol
3、vedinmanydifferentmetaboliteandiontransport,endocytosis,andcelldiffer-functionsdependingontheirlocationinthecell.entiationandproliferation.AlltheseprocessesinvolveCharacterizationofthemembraneproteomecanalargenumberofproteinswithhighlydiversestructuresbringnewi
4、nsightstothefunctionofdifferentplantandfunctions.membranesystemsandthesubcellularcompartmentsThedegreeofassociationofproteinswithamembranewheretheproteinsarefound.Plantmembranepro-varies.Someproteinsareembeddedinthemembranelipidteomicscanalsoprovidevaluableinfo
5、rmationaboutcore,whileothersaremoreperipheral,andassociatedbyplant-specificbiologicalprocesses.Despiterecentad-reversibleinteractionswitheitherlipidsorothermembranevancesintheseparationandtechniquesfortheanal-proteins(Marmagneetal.,2004).Plantmembraneproteo-ysis
6、ofplantmembraneproteins,characterizationofmicscangivevaluableinformationonplant-specificpro-theseproteins,especiallythehydrophobicones,isstillcesses;however,thechallengeforproteomicsistofindchallenging.Inthisreview,plantmembraneproteo-waysofextractingandidentifyi
7、ngtheentiresetofmostlymicsdata,compiledfromtheliteratureonArabidop-sisthaliana,aredescribed.Inaddition,initialattemptshydrophobicplasmamembraneproteins.towardsdeterminingthephysiologicalsignificanceProteomeanalysesatthelevelofsubcellularstructuresofsomeproteinsi
8、dentifiedfrommembraneproteomicsrepresentananalyticalstrategythatcombinesclassicalinricearealsodescribed.biochemicalfractionationmethodstoenrichforparticularcompartmentsandtoo
