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1、中国农业科学2004,37<4):477-483ScientiaAgriculturaSinica甘蓝型油菜SSR,ISSR标记的遗传多样性及其与杂种表现的关系沈金雄’，’，傅廷栋’，杨光圣’(‘中国农业科学院油料作物研究所，武汉430062;1华中农业大学作物遗传改良国家重点实验室，武汉430070)摘要:利用SSR,ISSR标记技术分析了3个甘蓝型油菜自交不亲和系及22个不同来源的父本品种的遗传多样性，及其与这些亲本按NCII法配制杂种的产量性状表现及杂种优势的关系。结果表明，墓于遗传距离的UPGMA(非加权类平均法)聚类法可将25个亲本材料分为6组
2、，SI-1300,SI-1320各为一组，来源于中国的2个父本品种为一组，SI-1310与来源于国外的其它品种分属另3组。这种分组结果与材料的来源及系谱关系墓本一致。各组间亲本在产量性状、种子品质性状及物候期性状上存在明显差别。SSR,ISSR标记的遗传距离与杂种单株产量极显著乓相关，但决定系数较小。SSR及ISSR标记遗传距离难以预测甘蓝型油菜杂种产量及其优势.关键词:甘蓝型油菜;遗传多样性;杂种表现;SSR;ISSRRelationshipBetweenHybridPerformanceandGeneticDiversityBasedonSSRand
3、ISSRinBrassicanapusL.SHENJin-xiong',2,FUTing-dongz,Guang-sheng2('OilCropsResearchInstitute,ChineseAcademyofAgriculturalSciences,Wuhan430062;'NationalKeyLaboratoryofCropGeneticImprovement,HuazhongAgriculturalUniversity,Wuhan430070)Abstract:Toinvestigatetherelationshipbetweengeneti
4、cdistance(GD)andhybridperformance,SSR(microsatellite)andISSR(intro-simplesequencerepeat)wereemployedtodetectthegeneticdiversityof3doublelowself-incompatiblelinesand22maleparentvarietiesinBrassicanapusfromdifferentorigins.HybridswereproducedinaNCIImatingdesignbyhand-pollination.Re
5、sultsindicatedthat25parentvarieties(lines)couldbedividedintosixgroupsbyUPGMA(un-weightedpairgroupmathematicsaverage)clusteringbasedonGD.SI-1300andSI-1320couldbesinglyclusteredintoonegroup,respectively.MalevarietiesfromChinacouldbedividedintoanothergroup.SI-1310andmalevarietiesfro
6、mforeigncountriescouldbedividedintootherthreegroups.Thegroupingwasgenerallyconsistentwithparentalpedigreesandgeographicalorigins.Significantdifferencesinyield,qualityandphenologicalperiodtraitswereobservedamongtheseparentgroups.Althoughhybridyieldperplantshowedsignificantpositive
7、correlationwithgeneticdistancebasedonSSRandISSRmarkers,butthedeterminationcoefficients(R2)werelow.ItappearedtobeunsuitableforusingthegeneticdistancebasedonSSRandISSRmarkerstopredictheterosisandhybridperformanceinBrassicanapus.Keywords:BrassicanapusL;Geneticdiversity;Hybridperform
8、ance;SSR;ISSR油菜是我国乃至全世界广泛种植的油料作物。106ha，总
