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1、圆园16,35(3):432-439农业环境科学学报2016年3月允燥怎则灶葬造燥枣粤早则燥鄄耘灶增蚤则燥灶皂藻灶贼杂糟蚤藻灶糟藻张云芳,陈楚,陈晨,等.镉胁迫对青菜幼苗某些生理特性以及基因组多态性的影响[J].农业环境科学学报,2016,35(3):432-439.ZHANGYun-fang,CHENChu,CHENChen,etal.EffectsofcadmiumstressesonphysiologicalcharacteristicsandgenomicDNApolymorphismofBrassicachinensisL.seedlings[J].允燥怎则灶葬造
2、燥枣粤早则燥-耘灶增蚤则燥灶皂藻灶贼杂糟蚤藻灶糟藻,2016,35(3):432-439.镉胁迫对青菜幼苗某些生理特性以及基因组多态性的影响张云芳,陈楚,陈晨,束良佐(淮北师范大学生命科学学院,安徽省资源植物生物学重点实验室,安徽淮北圆猿缘园园园)摘要:以青菜品种“苏州青”为试材,采用生理生化方法和随机扩增多态性DNA(RAPD)技术,研究了镉(Cd)胁迫对青菜幼苗相关生理特性以及基因组多态性的影响。结果表明:不同浓度(员、缘、员园、圆0、40mg·L-1)的Cd处理20d后,青菜幼苗的鲜重、根伸长受到显著抑制,叶片的叶绿素以及蛋白质含量下降,丙二醛含量和细胞膜透性增加。选
3、用8条寡核苷酸引物(10bp)对青菜幼苗基因组DNA进行RAPD扩增,对照组的RAPD图谱中可分辨出55条谱带,Cd胁迫后的RAPD图谱发生改变,呈现谱带的增加、缺失和荧光强度的改变,并且与Cd浓度剂量-效应相关。随着Cd浓度的增加,幼苗叶片基因组的模板稳定性逐步下降,并且RAPD多态性与青菜幼苗的生理指标具有显著的相关性。以上结果表明,利用RAPD技术获得的DNA多态性变化谱带可作为检测Cd对青菜毒性效应的生物标记物。关键词:青菜;镉胁迫;生理特性;RAPD;DNA多态性中图分类号:X503.231文献标志码:A文章编号:1672-2043(2016)03-0432-08
4、doi:10.11654/jaes.2016.03.004EffectsofcadmiumstressesonphysiologicalcharacteristicsandgenomicDNApolymorphismofBrassicachinensisL.seedlingsZHANGYun-fang,CHENChu,CHENChen,SHULiang-zuo(SchoolofLifeSciences,HuaibeiNormalUniversity,AnhuiKeyLaboratoryofResourcesandPlantBiology,Huaibei235000,Chin
5、a)Abstract:Cadmium(Cd)isatoxictracepollutantthatcaneasilybetakenupbyvegetablecrops.TheeffectsofCdstressesonsomephysio原logicalcharacteristicsandgenomicDNApolymorphisminBrassicachinensiscultivar"Suzhouqing"seedlingswerestudiedbyrandomam原plifiedpolymorphicDNA(RAPD)techniqueandphysiologicaltes
6、ts.Resultsshowedthattreatingplantswith1,5,10,20and40mg·L-1Cdfor20daysinhibitedfreshweightandrootelongationofseedlings,decreasedchlorophyllcontentandtotalsolubleproteincontent,butin原creasedmalondialdehydecontentandelectrolyteleakage.Using8oligonucleotideprimers(10bp)toamplifythegenomicDNAof
7、theseedlingsbypolymerasechainreaction,55clearRAPDbandsweredetectedinnormalseedlings.Comparedwiththecontrol,theRAPDprofilesofCd-exposedseedlingsshowednewbands,lostnormalbands,alteredbandintensities.Inaddition,DNApolymorphismwasdose-dependent.ThegenomicDNAtempla