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1、牙体牙髓牙周病学杂志(ChinJConservDent)2009,19(5)·271·五倍子牙周缓释凝胶对LPS介导HPDLFs分泌MMP-3的影响11112祝磊,唐荣银,张海欧,张安生,杨素芳(1.第四军医大学口腔医学院,陕西西安710032;解放军总政治部门诊部口腔科,北京100011)[摘要]目的:探讨五倍子牙周缓释凝胶对内毒素(LPS)介导人牙周膜成纤维细胞(HPDLFs)分泌基质金属蛋白酶-3(MMP-3)的影响。方法:体外培养HPDLFs,运用ELISA分别检测在LPS作用下以及在LPS加不同时间五倍子凝胶释放液作用下HP
2、DLFs分泌MMP-3的量。结果:与空白对照组相比,50μg/mL的LPS即能明显促进HPDLFs分泌MMP-3(P<0.05),100μg/mL的LPS促进作用更显著;加入五倍子凝胶释放液后,各时间点凝胶释放液组MMP-3的量均明显低于LPS组(P<0.05),而且,各时间点凝胶释放液组之间亦有显著性差异(P<0.05),其中,2h释放液组MMP-3最低,相对于LPS组,对MMP-3的抑制率为75.77%,随着释放时间的延长,抑制作用逐渐减弱,但第7天释放液仍有32.26%的抑制率。结论:五倍子牙周缓释凝胶可有效抑制LPS介导HPD
3、LFs分泌MMP-3,抑制作用可持续7d。[关键词]五倍子牙周缓释凝胶;人牙周膜成纤维细胞;基质金属蛋白酶-3;内毒素[中图分类号]R781.4[文献标识码]A[文章编号]1005-2593(2009)05-0271-04[牙体牙髓牙周病学杂志,2009,19(5):271]Effectsofperiodontalsustained-releasedgelofChinesenutgallontheexpressionofMMP-3inhumanperiodontalligamentfibroblastsinducedbylipopol
4、ysaccharide3ZHULei,TANGRong-yin,ZHANGHai-ou,etal3(SchoolofStomatology,FourthMilitaryMedicalUniversity,Xi′an710032,China)[Abstract]AIM:Todetecttheinfluenceofaperiodontalsustained-releasedgelofChinesenutgallonthesecretionofMMP-3inhumanperiodontalligamentfibroblasts(HPDLFs
5、)inducedbylipopolysaccharide(LPS).METHODS::HPDLFswereculturedinvitroandtreatedwithLPSorwithLPS+Chinesenutgallsolutionsreleasedfromthegelatdifferenttimepoints.TheamountsofMMP-3secretedbyHPDLFsweredetectedbyELISA.RE2SULTS:WhenthegroupstreatedbyLPSwerecomparedwiththeblankg
6、roup,significantincreases(P<0.05)inMMP-3levelwereobservedinthegroupstreatedbyLPSoftheconcentrationfrom50to100μg/mL.AftertreatedwithChi2nesenutgallsolutionsreleasedfromthegel,theamountofMMP-3ofallthegroupsweresignificantlylowerthanthatoftheLPSgroup(P<0.05).Significantdif
7、ferenceswerefoundamongallthegroups,theamountofMMP-3secretionofHPDLFstreatedwiththesolutionreleasedfromthegelinthefirst2hourswasthelowest.ComparedwiththeLPSgroup,theinhibitionratioofMMP-3ofthissolutionwas75.77%.Butwiththeextendingofreleasingtime,theinhibitoryac2tionwaswe
8、akeninggradually,however,theinhibitionratiostillremainedashighas32.26%onthe7thday.CONCLU2SION:Theperiodontalsu
