测序通用引物序列

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1、测序通用引物序列常见载体的测序引物：PrimerofVector:Vector：Primer(F)；Primer(R)pACTT7T3pACT2GAL4ADpACT2-RpAS2-1GAL4BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/M13-M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA1301(1300)P1P2pCAMBIA2300M13R(-48)M13F(-47

2、)PCANTB5ES1/M13RS6pCAT3-enhancerRVP3此载体无反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025在T7前面）BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCIT7（17Base）此载体无反向引物pCI-neoT7（17Base）T3pCMS-EGFPT7T3pCMV-3Tag-4AT3/

3、PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpCMV5FpCMV5RpCMV-MYC/HApCMV-FpCMV-RpCMV-SportM13F/T7SP6/M13RpCMV-Tag(KAN+)T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T7BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N

4、-3’(距离很近,一般不用)pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3’pDSRED-N1(KAN+)pEGFP-N-5’PDSRED-N-RpECFP-CpEGFP-C-5’pECFP-C-3′pECFP-N1pEGFP-N-5’pEGFP-N-3’pEF/myc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5’pEGFP-C-3′pEGFP-N(KAN+)pEGFP-N-5’pEGFP-N-3’pENTR/D-TOPO(KAN

5、+)M13FM13RpET-*(His)(KAN+)T7T7TerpET22(a,b,c)(AMP+)T7T7TerpET28,30,24,49(KAN+)T7T7TerpET32(a,b,c)(AMP+)T7/S.tagT7TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7TerpEYFP-N1pEGFP-N-5’pEGFP-N-3’pEYFP-C1pEGFP-C-5’pEGFP-C-3′pFLAG-CMVpFLAG-CMV-FpFLAG-CMV-

6、RpGAD424GAL4AD3′ADpGADGHGAL4AD3′ADpGADT7-RecGAL4AD/T73′ADpGAPZa-Aa-FACTOR3′AOXpGBKT7(Kana+)T73′BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5’pGEX-4T-3’pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)PinpointTMVectorPinpointPrimerSP6pIRESPIRES-F/T7T3/PI

7、RES-R(J40720)pIRES2-EGFPpIRES2-EGFP.P5’pIRES2-EGFP.P3’pLXSNpLXSN-FpLXSN-RpMAL-c2EMalEprimerM13F(-47)pMAL-C2xP5’P3’pMAL-p2XMalEprimerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+、ZEO+）5’AOX/a-factor3’AOXpPICZa5′AOX/PPICZa-F3′A

8、OXpPROEXHTAM13R(-48)无反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSilencer3.1-H1hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSport1SP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(优先使用M13F）T3/M13RpT-AdvM13F/T7M13RpTARGETTM