Visualizing real-time influenza virus infection transmission and protection in ferrets

《Visualizing real-time influenza virus infection transmission and protection in ferrets》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、ARTICLEReceived19May2014

2、Accepted23Jan2015

3、Published6Mar2015DOI:10.1038/ncomms7378OPENVisualizingreal-timeinfluenzavirusinfection,transmissionandprotectioninferretsErikA.Karlsson1,*,VictoriaA.Meliopoulos1,*,ChandraSavage2,BrandiLivingston2,AndrewMehle3

4、&StaceySchultz-Cherry1Influenzatransmissionefficiencyinferretsisvitalforrisk-assessmentstudies.However,theinabilitytomonitorviralinfectionandtransmissiondynamicsinrealtimeonlyprovidesaglimpseintotransmissibility.Hereweexploitareplication-competentinfluen

5、zareportervirustoinvestigatedynamicsofinfection/transmissioninferrets.BioluminescentimagingofferretsinfectedwithA/California/04/2009H1N1virus(CA/09)encodingNanoLuc(NLuc)luciferaseprovidesthefirstreal-timesnapshotofinfluenzainfection/transmission.Lumines

6、cenceintherespiratorytractandinlesswell-characterizedextra-pulmonarysitesisobserved,andimagingidentifiesinfectionsinanimalsthatwouldhaveotherwisebeenmissedbytraditionalmethods.Finally,thereportervirussignificantlyincreasesthespeedandsensitivityofvirolog

7、icalandserologicalassays.Thus,bioluminescentimagingofinfluenzainfectionsrapidlydeterminesintra-hostdissemination,inter-hosttransmissionandviralload,revealinginfectiondynamicsandpandemicpotentialofthevirus.Theseresultshaveimportantimplicationsforantivir

8、aldrugsusceptibility,vaccineefficacy,transmissibilityandpathogenicitystudies.1DepartmentofInfectiousDiseases,StJudeChildren’sResearchHospital,Memphis,Tennessee38105,USA.2AnimalResourceCenter,StJudeChildren’sResearchHospital,Memphis,Tennessee38105,USA.3

9、DepartmentofMedicalMicrobiologyandImmunology,UniversityofWisconsin-Madison,Madison,Wisconsin53706,USA.*Theseauthorscontributedequallytothiswork.CorrespondenceandrequestsformaterialsshouldbeaddressedtoA.M.(email:amehle@wisc.edu)ortoS.S.-C.(email:stacey

10、.schultz-cherry@stjude.org).NATURECOMMUNICATIONS

11、6:6378

12、DOI:10.1038/ncomms7378

13、www.nature.com/naturecommunications1&2015MacmillanPublishersLimited.Allrightsreserved.ARTICLENATURECOMMUNICATIONS

14、DOI:10.1038/ncomms7378easonaloutbreaksofinfluenzavi