金属硫蛋白的表面增强拉曼光谱研究

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1、中南大学硕士学位论文金属硫蛋白的表面增强拉曼光谱研究姓名：高宇申请学位级别：硕士专业：分析化学指导教师：向娟20080529硕士学位论文AbstractABSTRACTMetallothionein(MT)isatypeofmetalloproteinscharacterizedbyalargenumberofcysteineresidues．10Wmolecularweightandaremarkableabilitytocoordinatealargenumberoftransitionme

2、talionsintheformofmetal．thiolateclusters．Inrecentyeas．metallothioneindrawsmoreattentioninmedicalapplication．ItisrecognizedthatMTmaybecloselyrelatedtomanydiseases，particularlyAlzheimer'sdiseaseandotherneurodegenerativediseases．Therefore，thestudiesofdi

3、fferentmetal．bindingmechanismsandthestructuralchangesofmetallothioneinareofgreatsignificancestothepathologyofcertaindiseases．SERSiSanultrasensitiveanalyticalmethodtoprobethesurfacespeciesandCanprovidedetailedinformationofthestructureandchemicalcompos

4、itionofthespeciesonthemolecularlevel．Itiswell．knownthatenhancementeffectofSERSiscloselyconnectedwiththesuitablenanoscaleroughnessofthesubstratesornanoparticles．AndSERScanbeusedtounderstandthecomplicatedphysiologicalactivitiesofproteinonthemolecularle

5、vel．However，MThassmallRamanscatteringsection，andeasilybecomecatbonaceousdecompositionproductsbythelaser．ImpuritiesalsoconfuseSERSspectraofMT．Therefore，itisachallengetochoosesuitableSERSsubstrateandtheexperimentalconditionstoobtaincredibleSERSspectraw

6、ithgoodreproducibility，andthentostudythestructuralchangesofⅣ【Tcausedbyphysiologicalcharacteristicsonthemolecularlevel．Themajorworksandresultsinthethesisareoutlinedasfollows：1．SeveralmethodshavebeenexploredtoobtaintheSERSofmetallothionein．Resultsshowe

7、dthatSERSsubstratespreparedbyself-assemblynanoparticlesusuallyhadhigherSERS．activeandgoodreproducibilitywhereastheimpuritiesseriouslycoveredthesignalofMT．RoughenedAUsubstratewascleantoobtainreliableSERSsignal．ToobtaintheenhancedSERSsignalofMT．adirect

8、immobilizationofMTII硕士学位论文AbstractmoleculesontoroughenedAusubstratewasperformed．2．TheSERSspectraofMTabsorbeddirectlyonroughenedAusurfaceswasstudied．TheresultsindicatedthatMTwasadsorbedvia4寸H2grouponroughenedgoldsurfaces．Theadsorptionsiteofapo-MTonAuw