常用酶切位点的保护性碱基

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1、常用酶切位点的保护性碱基EnzymeOligoSequenceChain%CleavageLength2hr20hrAccIGGTCGACC800CGGTCGACCG1000CCGGTCGACCGG1200AflIIICACATGTG800CCACATGTGG10>90>90CCCACATGTGGG12>90>90AscIGGCGCGCC8>90>90AGGCGCGCCT10>90>90TTGGCGCGCCAA12>90>90AvaICCCCGGGG850>90CCCCCGGGGG10>90>90TCCCCCGGGGGA12>90>90BamHICGGATCCG8102

2、5CGGGATCCCG10>90>90CGCGGATCCGCG12>90>90BglIICAGATCTG800GAAGATCTTC1075>90GGAAGATCTTCC1225>90BssHIIGGCGCGCC800AGGCGCGCCT1000TTGGCGCGCCAA1250>90BstEIIGGGT(A/T)ACCC9010BstXIAACTGCAGAACCAATGCATTGG2200AAAACTGCAGCCAATGCATTGGAA242550CTGCAGAACCAATGCATTGGATGCAT2725>90ClaICATCGATG800GATCGATC800CCAT

3、CGATGG10>90>90CCCATCGATGGG125050EcoRIGGAATTCC8>90>90CGGAATTCCG10>90>90CCGGAATTCCGG12>90>90HaeIIIGGGGCCCC8>90>90AGCGGCCGCT10>90>90TTGCGGCCGCAA12>90>90HindIIICAAGCTTG800CCAAGCTTGG1000CCCAAGCTTGGG121075KpnIGGGTACCC800GGGGTACCCC10>90>90CGGGGTACCCCG12>90>90MluIGACGCGTC800CGACGCGTCG102550NcoIC

4、CCATGGG800CATGCCATGGCATG145075NdeICCATATGG800CCCATATGGG1000CGCCATATGGCG1200GGGTTTCATATGAAACCC1800GGAATTCCATATGGAATTCC2075>90GGGAATTCCATATGGAATTCCC2275>90NheIGGCTAGCC800CGGCTAGCCG101025CTAGCTAGCTAG121050NotITTGCGGCCGCAA1200ATTTGCGGCCGCTTTA161010AAATATGCGGCCGCTATAAA201010ATAAGAATGCGGCCGCTA

5、AACTAT242590AAGGAAAAAAGCGGCCGCAAAAGGAAAA2825>90NsiITGCATGCATGCA1210>90CCAATGCATTGGTTCTGCAGTT22>90>90PacITTAATTAA800GTTAATTAAC10025CCTTAATTAAGG120>90PmeIGTTTAAAC800GGTTTAAACC10025GGGTTTAAACCC12050AGCTTTGTTTAAACGGCGCGCCGG2475>90PstIGCTGCAGC800TGCACTGCAGTGCA141010AACTGCAGAACCAATGCATTGG22>90

6、>90AAAACTGCAGCCAATGCATTGGAA24>90>90CTGCAGAACCAATGCATTGGATGCAT2600PvuICCGATCGG800ATCGATCGAT101025TCGCGATCGCGA12010SacICGAGCTCG81010SacIIGCCGCGGC800TCCCCGCGGGGA1250>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGC2800GCGTCGACGTCTTGGCCATAGCGGCCGCGG301050ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA321075ScaIGAGTACTC8

7、1025AAAAGTACTTTT127575SmaICCCGGG6010CCCCGGGG8010CCCCCGGGGG101050TCCCCCGGGGGA12>90>90SpeIGACTAGTC810>90GGACTAGTCC1010>90CGGACTAGTCCG12050CTAGACTAGTCTAG14050SphIGGCATGCC800CATGCATGCATG12025ACATGCATGCATGT141050StuIAAGGCCTT8>90>90GAAGGCCTTC10>90>90AAAAGGCCTTTT12>90>90XbaICTCT