3.36 Cellular Therapies

《3.36 Cellular Therapies》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、3.36CellularTherapiesCTCarsonandNEmre,BDBiosciences–Pharmingen,LaJolla,CA,USACMcIntyreandTCFong,BDBiosciences,SanJose,CA,USA©2011ElsevierB.V.Allrightsreserved.3.36.1Introduction4123.36.2SourcesofCellsandClinicalApplicationsofCellTherapy4123.36.3Cell-BasedProductsforReconstructiveorStructuralRep

2、air4133.36.3.1CartilageRepair4133.36.3.2SkinRegeneration4133.36.3.3ProductsunderDevelopment4133.36.3.4OtherRelatedProductsinDevelopment4143.36.4CellularVaccines4143.36.5AdoptiveCellTherapies4153.36.6StemCell-DerivedTherapies4163.36.6.1BoneMarrow-DerivedNon-HSCTherapies4163.36.6.2UmbilicalCordan

3、dPeripheralBlood-DerivedStemCellTherapies4163.36.6.3Adipose-DerivedStemCells4193.36.6.4NeuralStemCells4193.36.6.5hESC-andhiPSC-DerivedTherapies4193.36.6.6EthicalConsiderations4193.36.7CellIsolationandProcessingMethods4203.36.7.1PhysicalMethods4203.36.7.2PlasticAdherence4223.36.7.3GradientCentri

4、fugation4223.36.7.4Elutriation4223.36.7.5Antibody-BasedMethods4223.36.7.6MagneticBeadSeparation4223.36.7.7Fluorescence-ActivatedCellSorting4233.36.7.8ExampleofaCellIsolationProcessforTregs4233.36.8Summary423References424GlossaryISSCR(InternationalSocietyforStemCellResearch)Anadoptivecelltherapy

5、Mostcommonlytheinfusionofaorganizationestablishedtopromoteandfosterthelymphocytepopulation(Tcells)intoapatientasanactiveexchangeanddisseminationofinformationandideasimmunotherapyfordisease.relatingtostemcells,toencouragethegeneralfieldofFACS(fluorescence-activatedcellsorting)Generally,aresearch

6、involvingstemcells,andtopromoteprofessionalmethodofidentifyingandselectingcellsofinterestandpubliceducationinallareasofstemcellresearchandbasedonthebindingoffluorescentantibodyreagentstoapplication.cell-surfacemarkersthatisuniqueforthatcelltype.Formesenchymalstromalcellsormultipotentstromalexam

7、ple,ahighlypurepopulationofTregsmaybecells(MSCs)Previouslydescribedasmesenchymalstem++lo+isolatedusingaCD4CD25CD127CD45RAprofile.cells,thesearefibroblast-likecellsthatwereoriginallyhumanembryonicstemcells(hESCs)Pluripotentcellsiso