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1、TransgenicRes(2008)17:121–132DOI10.1007/s11248-007-9085-zORIGINALPAPERSalttoleranceconferredbyoverexpressionofArabidopsis++vacuolarNa/HantiportergeneAtNHX1incommonbuckwheat(Fagopyrumesculentum)Li-HongChenÆBoZhangÆZi-QinXuReceived:19September2006/Accep
2、ted:16February2007/Publishedonline:31May2007ÓSpringerScience+BusinessMediaB.V.2007AbstractAgricultureproductivityisseverelyaf-esculentum)andtartarybuckwheat(Fagopyrumfectedbysoilsalinity.Onepossiblemechanismbytartaricum)areusedasfoodaroundtheworldwhic
3、hplantscouldsurvivesaltstressistocompart-(Bonafacciaetal.2003).Buckwheatseedscontainmentalizesodiumionsawayfromthecytosol.Inthe10–12.5%proteinandhavehighnutritionalvaluepresentwork,transgenicbuckwheatplantsoverex-(Greenwayetal.1980).Flavones,flavonoids
4、,sterols,++pressingAtNHX1,avacuolarNa/Hantiportergenefagopyrin,andthiamin-bindingproteinsinbuckwheatfromArabidopsisthaliana,wereregeneratedafterseedspossesspotentialeffectsintreatingsometransformationwithAgrobacteriumtumefaciens.chronicdiseases(Krefte
5、tal.2006).FlavonoidsareTheseplantswereabletogrow,flowerandaccumu-agroupofpolyphenolicplantsecondarymetaboliteslatemorerutininthepresenceof200mmol/lsodiumimportantforplantbiologyandhumannutrition.Inchloride.Moreover,thecontentofimportantnutrientstheseco
6、mpounds,flavonolsarepotentantioxidants,inbuckwheatwasnotaffectedbythehighsalinityofandtheirdietaryintakeiscorrelatedwithareducedthesoil.Theseresultsdemonstratedthepotentialriskofcardiovasculardiseases(Bovyetal.2002).Invalueofthesetransgenicplantsforagr
7、icultureuseinbuckwheat,themaincomponentofflavonoids(80%)salinesoil.isrutin,aflavonolglycoside(quercetin3-rhamno-sylglucoside).RutincanantagonizetheincreaseofKeywordsFagopyrumesculentumMoenchSaltcapillaryfragilityassociatedwithhaemorrhagicdis-++toleranc
8、eNa/HantiporterRutinHPLCease(Imetal.2003;Kreftetal.1999),reducehighbloodpressure(Abeywardenaetal.2001),decreasethepermeabilityofthebloodvessels,reducetheriskIntroductionofarteriosclerosis(Wojcickietal.1995),haveananti-oedemaeffectandantioxi
