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1、Biomaterials34(2013)10151e10159ContentslistsavailableatScienceDirectBiomaterialsjournalhomepage:www.elsevier.com/locate/biomaterialsAcancervaccinebasedonthemarineantimicrobialpeptidepardaxin(GE33)forcontrolofbladder-associatedtumorsabbcHan-NingHuang,VenugopalRajanbabu,Chieh-YuPan,Y
2、i-LinChan,a,**b,*Chang-JerWu,Jyh-YihChenaDepartmentofFoodScience,NationalTaiwanOceanUniversity,2,Pei-NingRoad,Keelung,TaiwanbMarineResearchStation,InstituteofCellularandOrganismicBiology,AcademiaSinica,23-10DahuenRoad,Jiaushi,Ilan262,TaiwancGraduateInstituteofMedicalSciences,Nation
3、alDefenseMedicalCenter,Taipei,TaiwanarticleinfoabstractArticlehistory:Themarineantimicrobialpeptide(AMP)GE33,alsoknownaspardaxin,possessesantimicrobialandReceived11July2013anticancerproperties,andmodulateshostsignaling.GE33hascytotoxiceffectsonmurinebladdercar-Accepted11September20
4、13cinoma(MBT-2)cells.Here,weinvestigatedthepotentialofGE33combinedwithinactivatedMBT-2asaAvailableonline24September2013cancervaccine.Thepresenceofupto12.5mgofGE33didnotinhibittheproliferationorendogenousnitrousoxide(NO)levelsofRAW264.7cells.However,thesecretionofMCP-1,IL-6,andIL-12
5、byKeywords:RAW264.7cellswasaffectedbyGE33.Weproceededtotesttheeffectivenessofthevaccinebyimmu-Pardaxinnizingmiceat7,14,and21daysofage,andinjectingliveMBT-2cellsonthe28thday.TumorgrowthbyAntimicrobialpeptidethe58thdaywasattenuatedinmicetreatedwiththevaccine,ascomparedtothecontrolgro
6、up.In-MBT-2BladdertumorductionofMBT-2specific-tumorantigenswasincreasedinmiceimmunizedwithourvaccine.CancervaccineFurthermore,activationofT-cellreceptors,cytotoxicT-cells,andNKcellswasenhanced,andtheseCytokinesshowedhighspecificityfortargetingtumorcells.Finally,immunizationcontrolled
7、excessrecruitmentofmonocytes,lymphocytes,T-helpercells,andNKcells,anddecreasedtheexpressionofVEGF.ThisreportprovidesempiricalevidencethatourGE33-basedvaccineenhancesantitumorimmunityinmice.Ó2013ElsevierLtd.Allrightsreserved.1.Introduction(ERK)andothersignalingmechanisms[6e8].Moreov
8、er,GE33exertsantitumoracti
