Basic Principles of Cell Culture

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1BasicPrinciplesofCellCultureR.IanFreshneyCentreforOncologyandAppliedPharmacology,CancerResearchUKBeatsonLaboratories,GarscubeEstate,Bearsden,GlasgowG611BD,Scotland,UK,i.freshney@ntlworld.com1.Introduction......................................................42.Ty

2、pesofCellCulture..............................................4
2.1.PrimaryExplantationVersusDisaggregation....................4
2.2.ProliferationVersusDifferentiation............................42.3.OrganotypicCulture.........................................72.4.S

3、ubstratesandMatrices......................................93.IsolationofCellsforCulture.......................................93.1.TissueCollectionandTransportation..........................93.2.BiosafetyandEthics..........................................103.3.Reco

4、rdKeeping.............................................113.4.DisaggregationandPrimaryCulture...........................114.Subculture........................................................114.1.LifeSpan....................................................124.2.Growt

5、hCycle...............................................124.3.SerialSubculture.............................................145.Cryopreservation.................................................146.CharacterizationandValidation.....................................166.1.

6、Cross-Contamination........................................166.2.MicrobialContamination.....................................166.3.Characterization.............................................186.4.Differentiation...............................................18Sour

7、cesofMaterials...................................................20References...........................................................21CultureofCellsforTissueEngineering,editedbyGordanaVunjak-NovakovicandR.IanFreshneyCopyright2006JohnWiley&Sons,Inc.3

1.INTRODU

8、CTIONThebulkofthematerialpresentedinthisbookassumesbackgroundknowledgeoftheprinciplesandbasicproceduresofcellandtissueculture.However,itisrecogni