表达谱芯片在医学中的应用

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1、Affymetrix表达谱芯片在医学基础研究中的应用复旦大学医学院郑汉城目录Affymetrix公司介绍基因芯片原理表达谱芯片实验流程表达谱芯片仪器平台表达谱芯片应用Affymetrix公司介绍TheGenomicsTimeLineFullimpactofgenomicknowledgeyettoberealizedCurrenteraisoneofknowledge-andtool-buildingAssociategenomicsequenceswithfunctionUnderstandimpactofp

2、olymorphismsFutureutilizationofgenomicknowledgemayallow:Fullyintegrative‘Systems’modelsforunderstandingdiseaseComprehensiveevaluationofdrugefficacyandsafetyPatientstratificationandclinicalmanagementPre-GenomicsCurrentEra(Transition)Post-GenomicsPre-~1980sNow

3、,next~15yearsFuture医学研究进入组学时代，基因芯片技术提供全基因组的视角MovingGeneChip®totheClinicAffymetrix致力于将技术应用于临床ResearchClinicClinicalApplicationsPre-clinical&ClinicalTrialsValidationandOptimizationMultipleChipFormatsHighThroughputAutomationDiscovery准确稳定方便完善Affymetrix基因芯片技术在临床

4、研究中的应用情况UnderstandingPatientResponseMechanismofActionMechanismofToxicityEfficacyinpre-clinicalDeterminedosageMolecularbasisofbiology/diseaseDiscoverandclassifydiseasesubtypesPredictdiseaseoutcomeIdentifytherapeutictargetsUnderstandingTherapyUnderstandingBiol

5、ogy/DiseaseClinicalefficacyClinicalsafetyTherapymodificationPatientstratification目前已发表21000篇文献Affymetrix系统及芯片的生产已经获得ISO国际认证在欧洲获得进入临床的许可美国食品和药物监督局（FDA）已经审批通过了AffymetrixGeneChip®System3000Dx(GCS3000Dx)——一种用于分析体外诊断（IVD）芯片的仪器系统Introduction:WhatGeneChipCanDo?Aff

6、ymetrix芯片技术囊括基因组转录组水平的研究热点EukaryoticCellDNARNADNAAnalysisRNAAnalysisGGCACGTGGCACGTGGAGGACentralAnnotation…andVisualizationOnePlatform–manyapplicationsOneGenechipSystemDNA--GenotypingFamilybasedlinkagestudies_10kWhole-genomediseaseassociation_SNP6.0Pharmacoge

7、netics_DMETChromosomalcopynumber_cyto2.7MPopulationgenetics_SNP6.0LinkageDisequilibrium_SNP6.0Resequencing_300kRNA--ExpressionAnalysisBiomarkerdiscovery_U133plus2.0MechanismofAction_U133plus2.0Transcriptionfactorbinding_promotertilingTranscriptomeAnalysis_ge

8、neST1.0DNAmethylation_promotertilingAlternativesplicing_exonAccessingtheGenomeMultipleapplicationsenabledonasingleplatformRNADNAWholeGenomeFocus500KU133TargetGenotypingCustomExpress™10K100KCusto