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1、JournalofEthnopharmacology113(2007)3544Genome-widebiologicalresponsefingerprinting(BioReF)oftheChinesebotanicalformulationISF-1enablestheselectionofmultiplemarkergenesasapotentialmetricforqualitycontrolJianhuiRonga,∗,RobertTiltonb,JiangangShenc,Kwan-MingNga,d,
2、ChangLiua,PaulKwong-HangTama,AllanSik-YinLaua,Yung-ChiChenge,∗aMolecularChineseMedicineLaboratory,LiKaShingFacultyofMedicine,TheUniversityofHongKong,21SassoonRoad,Pokfulam,HongKongbPhytoCeutica,Inc.5SciencePark,NewHaven,CT,06511,USAcSchoolofChineseMedicine,Th
3、eUniversityofHongKong,10ASassoonRoad,Pokfulam,HongKongdDepartmentofChemistryandOpenLaboratoryofChemicalBiologyoftheInstituteofMolecularTechnologyforDrugDiscoveryandSynthesis,TheUniversityofHongKong,PokfulamRoad,HongKongeDepartmentofPharmacology,YaleUniversity
4、SchoolofMedicine,333CedarSt.,SHMB226,NewHaven,CT06520-8066,USAReceived29July2006;receivedinrevisedform21December2006;accepted10January2007Availableonline20January2007AbstractQualitycontrolplaysacriticalroleintheprocessoftranslatingthetraditional/alternativeme
5、dicinesintomodernevidence-basedtherapies.Highperformanceliquidchromatography(HPLC)iswidelyappliedtoassessthechemicalcompositionofbotanicaldrugproducts.Thechromatographicfingerprintsorchemicalprofilesarecurrentlyusedasthedefactoqualitycontrolmetric.Asacomplement
6、tochemicalprofiles,abiologicalqualitycontrolassessmentoffersdistinctadvantages.Thisstudydescribesagenome-widebiologicalresponsefingerprinting(BioReF)approachtodefineasetofmarkergenesthatdefineasignaturepatternforaspecificbotanicalformulation.Thesemarkergenesarecho
7、senonthebasisofthelevelsoftheregulatedexpressionandtheinvolvementinthecellularsignalingpathways.Subsequently,qRT-PCRtechniqueisusedtosimultaneouslymonitorthegeneexpressionofmultiplemarkergenesinanefficientandquantitativemanner.Thissetofmarkergenesrepresentsthe
8、biologicalresponsesofhumancellstothechemicalcompositionofthebotanicaldrugthatcouldserveaspotentialqualitycontrolofbotanicaldrugsintermsoftheconsistencyofbiologicalactivities.Wedemonstrate
