Differential miRNA expression in Rehmannia

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1、Yangetal.BMCPlantBiology2011,11:53http://www.biomedcentral.com/1471-2229/11/53RESEARCHARTICLEOpenAccessDifferentialmiRNAexpressioninRehmanniaglutinosaplantssubjectedtocontinuouscropping†††††*YanhuiYang,XinjianChen,JunyingChen,HaixiaXu,JuanLiandZhongyiZhangAbs

2、tractBackground:TheproductivityofthemedicinallysignificantperennialherbRehmanniaglutinosaisseverelyaffectedafterthefirstyearofcropping.Whilethereissomeinformationavailabledescribingthephysiologicalandenvironmentalcausesofthisyielddecline,thereisasyetnodatareg

3、ardingthechangesingeneexpressionwhichoccurwhenthespeciesiscontinuouslycropped.Results:Usingamassivelyparallel(Solexa)DNAsequencingplatform,itwaspossibletoidentifyandquantifytheabundanceofalargenumberofR.glutinosamiRNAs.WecontrastedthemiRNAcontentoffirstyearcr

4、opplantswiththatofsecondyearcropones,andwereabletoshowthatof89conserved(belongingto25families)andsixnovelmiRNAs(sixfamilies),29oftheformerandthreeofthelatterweredifferentiallyexpressed.ThethreenovelmiRNAswerepredictedtotargetsevengenes,andthe29conservedones30

5、8genes.Thepotentialtargetsof32ofthesedifferentiallyexpressedmiRNAsinvolvedinthemaintranscriptionregulation,plantdevelopmentandsignaltransduction.AfunctionalanalysisofthedifferentiallyexpressedmiRNAssuggestedthatseveraloftheproposedtargetscouldbedirectlyorindi

6、rectlyresponsibleforthedevelopmentofthetuberousroot.Conclusion:WehavecompareddifferentialmiRNAsexpressioninthefirstyearcrop(FP)R.glutinosaplantsandsecondyearcrop(SP)ones.Theoutcomeidentifiessomepotentialleadsforunderstandingthemolecularbasisoftheprocessesunde

7、rlyingthedifficultyofmaintainingtheproductivityofcontinuouslycroppedR.glutinosa.BackgroundcropoverseveralseasonshavefailedtoovercometheRehmanniaglutinosaL.isaperennialherbaceousspeciesmajordeclineinproductivity,asthetubersareincreas-belongingtotheScrophularia

8、ceaefamily.Itseconomicinglyreplacedbyfibrousroots,whichareunabletoimportanceresultsfromthemedicinalactivitypresentindevelopintotubers[3,4].Muchofthepastresearchextractsofitstuberousroots[