合肥市西园新村小学校园文化建设工

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1、FYincipiesofRuorescenceSpectroscopyChemistryDepartmentXMU0123456■■■■■■■di^1didi-1u—-1—IntroductiontoRuorescenceIntroductionPhenomenonofRuorescenceEccitationandDeactivationofmoleculeModelsofMolecularemissionCharacteristicsofRuorescenceBnissionCharacteristicsofRuorouhoreFIuorescenceq

2、uenching17Resonaneeenergytransfer1.8TimesealeofFIuorescence19IntensityandConcentration110Ffuorouhore1.5CharacteristicsoffIuorophore>1.5.1Bccitationwavelength激发波长2ex>1.5.2Bnissionwavelength发射波长2ezn>1.5.3E(tinetion(absorption)coefficient吸光系数s>1.5.4Stokes5shift>1.5.5FluorescenceIifeti

3、me荧光寿命t>1.5.6HuorescenceciijantuiTiyield荧光量子产率①>1.5.7FluorescenceAnisotropy各项异性r>15.8HuorescenceFblarization荧光偏振pAbsorptionandemissionspectraEccitationandemissionspectraExcitationspectrumEmissionspectra入emThreedimensionspectrumThreedimensionspectraEC/xayItl&利V■，混令物的宝测警報度燙比光谓引自林竹光等人

4、的论文Fig；.6flxjperimcncad.contunuSfscctraufV&1bltkLV1.5.5Ruorescencelifetime•DefinitionLifetimeforSinglemolecule:thetimethemoleculespendsintheexcitedstatepriortoreturntothegroundstate・Averagelifetime:theaveragetimethemoleculespendsintheexcitedstatepriortoreturntothegroundstate.rAvera

5、gefluorescencelifetime1e•EqpressionSimplifiedJablonskidiagramLifetimerelaxation(10*12s)rEmissionrate:nrKnronradiativedecay；rate]IntrinsicornaturallifetimeT=厂+S1r°=7Thelifetimeofthefluorophoreintheabsenceofnonradiativeprocessesiscalledtheintrinsiclifetime.1.5.5fluorescencequantum

6、yield■DefinitionThefluorescencequantumyieldistheratioofthenumberofphotonsemittedtothenumberabsorbed■B(pressionr+knr■Fblationshipwithlifetime1•DaterminationComparisonwithastandard1-ChoseaStandardwithaquantumyield込2.Atdeterminetheabsorption(4S)ofthestandard3・At九ex=2/excitedthestandar

7、d,andintegratetheemissionspectrumofstandard,getF4.Repeat3thstepwiththeblanksolvent.Minustheemissionfromblank,getFs5.Repeat2-4thstepswiththesample,andgetAxandFx■ChooseStandardsEnoughabsorbancebothstandardandsampleatchosenexcitedwavelength.Moderatequantumyield・Examples1.0.05mol/Lsulf

8、ateofquinine,0=0.552.RuPy3