c小鼠皮肤炎症反应的实验研究

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1、-5O·中国血吸虫病防治杂志2012年第24卷第1期ChinJSchistoControl2012，Vo1．24，No．1[文章编号]1005．6661(2012)01-0050-04·论著·日本血吸虫热休克蛋白70诱导BALB／c小鼠皮肤炎症反应的实验研究杨洁，杨琳琳，孙希，郑焕钦，吕志跃，吴忠道[摘要]目的观察重组日本血吸虫热休克蛋白70(rSj648／hsp70)诱导BALB／c小鼠皮肤炎症反应的变化情况。方法BALB／c小鼠经腹部皮内分别免疫注射LPs(20只)、rSj648／hsp70(100只)，同时设PBS处理组为空白对照。通过苏木素伊红(HE)染色观察免疫后第1、2．4、7

2、天小鼠腹部皮肤的炎症反应变化，并利用Rea1．timePCR方法检测不同时间点各组小鼠皮肤组织IFN．mRNA的表达水平。结果通过HE染色观察发现LPS组小鼠腹部皮肤组织在免疫后第1天炎症细胞浸润明显，之后炎症反应开始逐渐减轻；与LPS组相比较，rSj648／hsp70组小鼠腹部皮肤炎症反应强度大，持续时间长，直至免疫后第7天炎症反应仍明显。Real—timePCR结果显示，免疫后第1天，LPS组和rSj648／hsp70组小鼠皮肤组织的IFN一mRNA表达水平均升高，之后LPS组逐渐降低，而rsj648msp7O组直至免疫后第7天仍持续高表达。结论rSj648／hsp70能使小鼠皮肤出现

3、较强的以诱导产生IFN．为主的Thl优势免疫应答，进一步提示了HSP70高表达可能与致弱尾蚴诱导宿主产生抗感染免疫力有关。[关键词]日本血吸虫；热休克蛋白70；炎症反应；皮肤；免疫应答；小鼠[中图分类号]R383．24[文献标识码]ASkininflammationresponsesinducedbyheatshockprotein70derivedfromSchistosoma]aponicuminBALB／cmiceynJic，YangLin—lin，SunXi，ZhengHuan—qin，LvZhi-yue，WuZhong-daoDepartmentofParasitology，Zh

4、ongshanSchoolofMedicine，SunYat-SenUniversity，KeyLaboratoryofTropicalDiseasesControl，MinistryofEducation，Guangzhou510080，ChinaCorrespondingauthor[Abstract]ObjectiveTostudytheskininflammationresponsesinducedbyheatshockprotein70(rSj648／hsp70)fromSchis—tosomajaponicuminBALB／cmice．MethodsBALB／cmicewere

5、injectedwith20gofLPSinabdominalskinor100gofrSj648／hsp70，meanwhile，thePBS-treatedgroupwassetasblank．Ondays1，2，4，7aftertheimmunization，dynamicchangesofinnammationwereobservedbystainingwithHE；andtheIFN．mRNAexpressionwasdetectedbyreal-timePCR．ResultsOnday1，theinflammationoftheskinsderivedfrommiceinjec

6、tedwithLPSwasobviously，andthenfellofgraduallyafterday1．ComparedwiththeLPS—treatedgroup，theinflammationresponsesinducedbySj648／hsp70werelongerandmoreintensiveuntilday7．Thereal-timePCRshowedthattheIFN一-ymRNAwasexpressedhighlyinbothLPS—treatedgroupandSj648／hsp70一treatedgroup，thenitfellofintheLPS—trea

7、tedgroup，buttheIFN一^ymRNAwasstillexpressedhigherintheSj648／hsp70一treatedgroupuntilday7．ConclusionsHeatshockprotein70(rSj648／hsp701induceshighprotectionagainstschistosome-infectioncontribut—ingtopredominantThlreac