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1、摘要I-II1前言12实验材料32.1药材32.2菌种32.3试剂与仪器33实验方法33.1屮药香薰消毒剂的组方及制备33.2香薰中药消毒液抑菌活性试验43.3最低抑菌浓度(MIC)的测定53.4病房消毒试验64实验结果74.1香薰屮药消毒液抑菌活性试验74.2香薰中药消毒剂最低抑菌浓度(MIC)的测定94.3病房消毒试验105讨论10参考文献12综述14致谢21香薰中药消毒剂的抑菌活性研究摘要:目的制备香薰中药消毒剂,观察其对病房常见菌的抑制作用。方法采用K-B纸片扩散法观察香薰中药消毒液对金黄色葡萄球菌、铜绿
2、假单胞菌、大肠杆菌、肺炎链球菌、粪肠球菌及肺炎克雷伯菌等病房常见菌的抑制作用;采用平板法涂布研究了供试菌的最低抑菌浓度(MIC);采用自然沉降法,观察香薰中药消毒液对病房消毒后lh和通风后lh的消毒效果。结果香薰中药消毒液对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、肺炎克雷伯菌有较好的抑制作用,尤其对金黄色葡萄球菌和大肠杆菌最为敏感。香薰中药消毒剂对金色葡萄球菌、大肠埃希菌、绿脓假单胞菌、肺炎克雷伯菌的MIC分别为:2・50mL/L、5.OOmL/L、40mL/L、20mL/Lo用于病房消毒,在消毒lh后及通风lh
3、后仍保持较好的消毒效果,杀菌率均达75%以上,并且消毒效果与3%过氧化氢相当。结论香薰中药消毒液对病房常见菌有较好的抑制作用,并且气味芳香,值得推广。关键词:香薰屮药消毒剂;病房消毒;最低抑制浓度;抑菌活性注:本论文题目来源于教师的市级科研项目,项目编号为:J2012070。TostudytheAntimicrobialActivityofFragrantdisinfectantDisinfectionAbstract:ObjectiveToprepareFragrantdisinfectantDisinfect
4、ionandobserveitsinhibitoryeffectonwardcommonbacteria.MethodsToobservetheinhibitoryeffectofaromatherapydisinfectantisolatesofStaphylococcusaureus,Pseudomonasaeruginosa,Escherichiacoli,EnterococcusfaecalisandStreptococcuspneumoniae,KlebsiellapneumoniawardswithK
5、-Bdiskdiffusionmethod.Theminimuminhibitoryconcentration(MIC)byplatespreadandantimicrobialactivitybyplatediffusetotestedbacteria.Usingasthemethodoffallingdownnaturallytoobservethedisinfectioneffectofaromatherapyliquiddisinfectantforwardlhandventilationafterlh.
6、ResultsAromatherapydisinfectanthasgoodinhibitoryeffectonStaphylococcusaureus,Pseudomonasaeruginosa,Escherichiacoli,Klebsiellapneumoniae,especiallyforStaphylococcusaureusandEscherichiacoliwasthemostsensitive.theminimuminhibitoryconcentration(MIC)toStaphylococc
7、usaureus,Pseudomonasaeruginosa,Escherichiacoli,Klebsiellapneumoniaewas2.5mL/L、5.0mL/L^40mL/Land20mL/L.Forward,stillmaintainagooddisinfectionefficacyindisinfectionoflhandventilationafterlh,thesterilizationratereachedabove75%,andthedisinfectioneffectand3%Hydrog
8、enperoxide.ConclusionAromatherapydisinfectanthasgoodeffectoncontrollingunitcommonbacteria,andsmellfragrant,worthyofpromotion.Keywords:Fragrantdisinfectantdisinfection;Warddisinfection;Min