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1、CentralJSMClinicalOncologyandResearchShortCommunication*CorrespondingauthorYoshioNaya,DepartmentofUrology,MeijiUniversityofIntegrativeMedicine,Hiyoshi-cho,Nantan,Kyoto629-ImagingofUrological0392Japan,Tel:81-771-72-1221;Fax:81-771-72-0234;Email:Submitted:11December2016CancerMorphologybyUsi
2、ngAccepted:25February2017Published:21March2017Probe-BasedConfocalLaserCopyright©2017Nayaetal.EndomicroscopywithNewOPENACCESSKeywordsContrastAgent-Preliminary•Endomicroscopy•Laseropticalfiber•UrothelialcarcinomaStudyforNewTURBTTechnique-•EthacridineYoshioNaya1,2*,EiichiKonishi3,NatsukiTaka
3、ha1,MasakatsuOishi2,TakashiUeda2,FumiyaHongo2,TsuneharuMiki2,andOsamuUkimura21DepartmentofUrology,MeijiUniversityofIntegrativeMedicine,Japan2DepartmentofUrology,KyotoPrefecturalUniversityofMedicine,Japan3DepartmentofPathology,KyotoPrefecturalUniversityofMedicine,JapanAbstractObjective:Too
4、bservethenucleusoftumorcellusingprobe-basedconfocallaserendomicroscopy(pCLE),weexaminedethacridineasnewcontrastagent.Recently,usefulnessofpCLEbyusingfluoresceinfordetectionofurothelialcarcinomawasreported.Fluoresceindidnotrevealcellnucleusandcytoplasm.Inthisstudy,weexaminedethacridineforp
5、CLEtoobservethenucleiofurothelialcarcinomacellsexvivo.Methods:3sampleswereobtainedfromTURBTspecimens.Samplesfromfreshlyexcisedurothelialcancerouslesionwereretrievedfromresectedtissue.Thesampleswereplacedontheglassslide,immersedimmediatelyinethacridinesolution(0.01%insaline)for60secs,andsu
6、bsequentlyrinsedinnormalsalinesolution,andpCLEimagingwasperformed.Afterobservation,thesampleswerefixedbyformalinontheglassslide,followedbyplacementofacoverglass,andstainedbyhematoxylin-eosin.TheimagingofpCLEwascomparedwithhistologicfindingsunderthesamehorizontalview.Results:All3patientswi
7、thhigh-gradeurothelialcarcinoma,stainednucleiwereobserved.ThemorphologicalpatternsofpCLEwerelikethoseofhistopathologicalstudy.Conclusion:pCLEwithethacridinecanclearlydetectthecancerouslesion.pCLEbyusingintravesicalethacridineinstillationmightbeusefulandlessinvasive.
