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1、维药毛菊苣水提取物抗炎作用实验探究［摘要］目的探讨维吾尔族习用药材毛菊苣水提取物(MS)的抗炎作用。方法体外采用脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7细胞，建立炎症模型。采用四甲基偶氮坐盐(MTT)法检测MS的细胞毒性作用，采用Griss法检测细胞上清中NO的生成量，酶联免疫吸附测定(ELISA)检测细胞上清中白细胞介素-l&beta；(IL-l&beta；).肿瘤坏死因子-&alpha；(TNF-&alpha；)和白细胞介素TO(IL-10)的水平；在体实验采用冰醋酸致小鼠腹腔毛细血管通透性亢进模型，观察MS的体内抗炎作用。结果体外实验，与空白对照组
2、比较，MS250&mu；g/mL以下浓度对RAW264.7细胞生长无影响。MS15.625、31.25.62.5.125、250&mu；g/mL浓度均可明显抑制LPS诱导的RAW264.7细胞上清中NO和ILT0的生成(P<；0.01),此外，250&mu；g/mL剂量组可显著抑制IL-l&beta；的分泌(P<；0.01),且随着MS浓度的升高，对TNF-&alpha；分泌的抑制作用逐渐增强；在体实验，与模型组比较，MS130、260mg/kg剂量组对冰醋酸致小鼠腹腔毛细血管通透性亢进均有显著的抑制作用(P<；0.01),抑制率分别达到49%和57
3、%o结论MS具有显著的抗炎活性。［关键词］毛菊苣；水提取物；抗炎作用；RAW264.7细胞；白细胞介素-l&beta；:白细胞介素-10；肿瘤坏死因子-&alpha；［中图分类号］R285.5［文献标识码］A［文章编号］1674-4721(2016)08(c)-0027-04［Abstract］ObjectiveToinvestigatetheanti-inflammatoryeffectsofCichoriumglandulosumBoiss.etHuetaqueousextracts(MS)・MethodsInvitro,lipopolysaccharide
4、wasusedtoinduceRAW264.7cellstobuildmodelofinflammation.TheeffectofMSoncelltoxicwasdetectedbymethylthiazolyltetrazoliumassay(MTT)・TheNOgenerationincellsupernatantwasdetectedbyGrissmethod,andthelevelsofinterleukinT&beta；(IL-l&beta；),tumornecrosisfactor-&alpha；(TNF-&alpha；)andinterleuki
5、n10(IL-10)incellsupernatantweredetectedbyEnzyme-linkedimmunosorbentassay(ELISA).Invivo,themodelsoftheincreaseofcapillarypermeabilityinmiceinducedbyaceticacidwereusedtoevaluatetheanti-inflammatoryeffectofMS.ResultsInvitro,comparedwiththecontrolgroup,MS(&le；250&mu；g/mL)hadnoinfluenceon
6、theproliferationofRAW264.7cells.MS15.625,31.25,62.5,125,250&mu；g/mLsignificantlydecreasedthelevelsofNOandIL-10(P<；0.01)・Meanwhile,250&mu；g/mLMSsignificantlydecreasedthelevelsofIL-l&beta；(P<；0.01),andwiththeincreaseoftheconcentrationofMS,theinhibitiononTNF-&alpha；secretiongraduall
7、ystrengthened.Invivo,comparedwiththemodelgroup,130,260mg/kgMSsignificantlyinhibitedtheincreaseofabdominalcavitycapillarypermeabilityinmicecausedbyaceticacid(P&It；0.01),theinhibitionratewas49%and57%respectively.ConclusionMShassignificantanti-inflammatoryeffects.[Keywords]Cichoriumglan
8、dulosumBoiss
