Induced expression of defense-related genes in Arabidopsis upon infection with Phytophthora capsici.ppt

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1、Inducedexpressionofdefense-relatedgenesinArabidopsisuponinfectionwithPhytophthoracapsici234TheresearchpurposeandmeaningTheaimofthisstudywastodemonstratethatcamalexin,indoleglucosinolates(iGS)andsalicylicacid(SA)havearoleindefenseagainstP.capsici.Makeafurtherinvestigationabouttheroleofcamalexin-,iGS-

2、andSA-relatedpathwaysinthedifferentialinteractionbetweenArabidopsisandP.capsici.Thisstudycontributestotheresearchofthemachanismaboutplant'sdefenseagainstpathogenicbacteria.5Selectionofpathogens(LT263,LT123)andarabidopsis(Col-0,Ler-0)plug-inoculationwithLT263andLT123onCol-0andLer-0totalRNAextractionR

3、T-PCRreal-timePCRdataanalysisdiseasesymptomsexplainthedefensemechanismTechnologyroute6reseachmethods:plug-inoculation,real-timePCRexpectresults:ThearabidopsisgotinoculatedandtheDSIisCol-0/LT263＞Col-0/LT123,Ler-0/LT263＞Ler-0/LT123,Ler-0/LT263＞Col-0/LT263,Ler-0/LT123＞Col-0/LT123.Afterplug-inoculatio

4、n,thelevelofgeneshavechanges,respectively.Thegenes'transcriptlevels'foldchangecausedbyLT263ishigherthanthechangecausedbyLT123.7Thisstudyhasshowndifferentaccession-isolatecombinationsandfornearlyallmarkergenesexpressionwashigherincompatibleinteractionsascomparedwithincompatibleinteraction.However,the

5、expressionprofiles,donotrevealanyobviouscorrelationbetweenexpressionlevelsanddiseasephenotype.Thedefensemechanismissocomplexthatitisnoteasytogetitclearthoughtherearesomanystudiesandreseachesonit.Soitisnecesssarytohavefurtherandcomprehensiveresearchonthemechanisminthefuture.8Phytophthoracapsici:•apat

6、hogenwithabroadhostrange•particularlydevastatinginvegetablecropsworldwidecompatibleinteractions:infectionincompatibleinteractions:resistance9Defenseresponsesactivationofdefense-relatedgenesproductionofantimicrobialcompoundsPhytophthoracapsicicaninfectarabidopsisandrevealedinteractionspecificityamong

7、variousaccession-isolatecombinations.10LT263：colonizationofleafandroottissue,haustoriaformation,sporulationLT123:incompatbileinteractions--callosedeposition,accumulationofactiveoxygenspecies,celldeath