生物化学论文 英语 翻译.doc

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1、生物化学论文英语翻译浙江师范大学本科毕业设计（论文）外文翻译1本科毕业设计（论文）外文翻译2本科毕业设计（论文）外文翻译3本科毕业设计（论文）外文翻译4本科毕业设计（论文）外文翻译5本科毕业设计（论文）外文翻译原文一:CarotenoidBiosyntheticPathwayintheCitrusGenus:NumberofCopiesandPhylogeneticDiversityofSevenGeneJournalofAgricuLturalandFoodChemistry.2007,55(18)：7405^7417Thefirstobjectiveofthispaperwasto

2、analyzethepotentialroleofallelicvariabilityofcarotenoidbiosyntheticgenesintheinterspecifidiversityincarotenoidcompositionofCitrusjuices.Thesecondobjectivewastodeterminethenumberofcopiesforeachofthesegenes.Sevencarotenoidbiosyntheticgeneswereanalyzedusin呂restrictionfragmentlengthpolymorphism(RFLP

3、)andsimplesequeneerepeats(SSR)markers.RFLPanalyseswereperformedwiththegenomicDNAobtainedfrom25Citrusgenotypesusingseveralrestrictionenzymes.cDNAfragmentsofPsy,Pds,Zds,Lcyb,Lcy-e,Hy-b,andZepgeneslabeledwith[R-32P]dCTPwereusedasprobes.ForSSRanalyses,twoprimerpairsamplifyingtwoSSRsequencesidentifie

4、dfromexpressedsequeneetags(ESTs)ofLcy-bandHy-bgenesweredesigned.ThenumberofcopiesofthesevengenesrangedfromoneforLcy-btothreeforZds.ThegeneticdiversityrevealedbyRFLPandSSRprofileswasinagreementwiththegeneticdiversityobtainedfromneutralmolec(iLarmarkers.GeneticinterpretationofRFLPandSSRprofilesoff

5、ourgenes(Psyl,Pdsl,Lcy-b,andLcy-el)enabledustomakeinferencesonthephylogeneticoriginofallelesforthemajorcommercialcitrusspecies.Moreover;theresuLtsofouranalysessuggestthattheallelicdiversityobservedatthelocusofbothoflycopenecyclasegenes,Lcy-bandLcy-el,isassociatedwithinterspecificdiversityincarot

6、enoidaccum^iLationinCitrus.Theinterspec讦icdifferencesincarotenoidcontentspreviouslyreportedtobeassociatedwithotherkeystepscatalyzedbyPS^HY-b,andZEPwerenotlinkedtospecificallelesatthecorrespondingloci.KEYWORDS:Citrus;carotenoids;biosyntheticgenes;allelicvariability;phylogenyINTRODUCTIONCarotenoid

7、sarepigmentscommontoallphotosyntheticorganisms.Inpigment・proteincomplexes,theyactaslightsensorsforphotosynthesisbutalsopreventphoto-oxidationinducedbytoostronglightintensities.Inhorticulturalcrops,theyplayamajorroleinfruit,r