资源描述：
《冠状病毒Coronavirusl论文-1989 Comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、VirusResearch,13(1989)15-2815ElsevierVRR00490ComparativefeaturesofacoronavirusisolatedfromacheetahwithfelineinfectiousperitonitisJamesF.Evermann,JonathanL.Heeney2,AlisonJ.McKeimanandStephenJ.OBrien2’~e~~trnentofVeterinnlyC&nical~ediei~eandSurgery,CollegeofVeterin
2、ary~edjcine,WashingtonStateUniversity,Pullman,Wmhington,U.S.A.and2LAvmtoryofViralCarcinogenesis,NationalCancerInstitute,FrederickCancerResearchFacility,Frederick,MarylandU.S.A.(Accepted13January1989)Acoronaviruswhichwasisolatedfromacheetah(Acinonyxjubutur)thatsuc
3、cumbedtofelineinfectiousperitonitiswascharacterizedinvitro.Theviruswasdeterminedtobehighlycell-associatedwithCrandellfelinekidney(CrFK)cellsandwasroutinelyrn~~nedasapersistentinfection(CrFK83-4497).Thecheetahcoronaviruswascomparedwithothermembersofthefelinecorona
4、virusgroupincludingthefelineentericcoronavirus(FECV)79-1683andthefelineinfectiousperitonitisviruses(FIPV),79-1146,andUCD-1.Thecheetahcoronaviruswasdemonstratedtohavearestrictedhost-cellrangewithlimitedcytopathiceffect.IndirectimmunofluorescencewithantiseratoFIPVU
5、CD-1revealedtheconcentra-tionofviralantigensintheperinuclearregionofcellsinfectedwiththecheetahcoronavirus.Ultrastructuralstudiesofthecheetahcoronavirusindicatedalimitednumberofimmatureviralparticleswithincytoplasmicvesiclesandatthecellsurface.Thiswasincontrastto
6、electronmicroscopyresultsofFECV79-1683andFIPV79-1146,whichhadnumerousmaturevirusparticleswithinthecytoplasmicvesicles,aswellasatthecellsurface.Thecheetahcoronaviruswastentativelyplacedinthefelinecoronavirusfamilybaseduponitsantigenicreactivitybyimmunofluorescence
7、;however,thepossibilitythatitrepresentsauniquecoronavirusofcheetahsshouldnotbedismissedwithoutfurtheranalysesatthehostandgenomiclevels.Cheetahcoronavirus;Felineinfectiousperitonitisvirus;FelineentericcoronavirusCorrespondenceto:J.F.Evermann,DepartmentofVeterinary
8、ClinicalMedicineandSurgery,CollegeofVeterinaryMedicine,WashingtonStateUniversity,Pullman,WA99163-7010,U.S.A.0~68-1702/89/$03.5001989ElsevierSciencePublishersB.
