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1、J.gen.Virol.(1986),67,1443-1448.PrintedinGreatBritain1443Keywords:coronavirusIBV/avianIBV/spikeprotein~cellattachmentCoronavirusIBV:RemovalofSpikeGlycopolypeptideS1byUreaAbolishesInfectivityandHaemagglutinationbutNotAttachmenttoCellsByDAVIDCAVANAGH*ANDPHILIPJ.DAVISHoug
2、htonPoultryResearchStation,Houghton,Huntingdon,Cambs.PE172DA,U.K.(Accepted8April1986)SUMMARYUreahasbeenusedtoremovetheS1spikeglycopolypeptidefromavianinfectiousbronchitisvirus(IBV)strainsM41andBeaudette,withoutremovingthe$2spike-anchoringglycopolypeptide.Reductionofthe
3、pHto2-9didnotcausereleaseofS1althoughsomeS1wasreleasedspontaneouslyfromIBVBeaudetteatpH7.4.VirusthatlackedS1wasnolongerinfectiousorabletocausehaemagglutination(HA).However,radiolabelledIBVthatlackedS1attachedtoerythrocytesandchickkidneycellstothesameorsimilarextentasdi
4、dintactvirus.TreatmentofIBVwithaphospholipaseCpreparation,requiredtomakeIBVcauseHA,didnotincreasebindingofIBVtoerythrocytes.TheresultsindicatethatwhiletheattachmenttocellsofvirusthatlacksS1isqualitativelydifferentfromthatofintactvirus,thedeclineininfectivityistheconseq
5、uenceofthelossofsomeotherspikefunction.Avianinfectiousbronchitisvirus(IBV)causeseconomicallyimportantdiseaseinthedomesticfowl.Ofthethreestructuralproteins,one,thenucleocapsid(N)protein,iswithinthelumenofthevirusformedbythevirusmembranewhiletheothertwoproteins,themembra
6、ne(M)andspike(S)glycoproteins,arepartiallyexposedattheoutervirussurface.Onlyasmall,glycosylatedportionofMproteinappearstobeexposedatthevirionsurface(Boursnelletal.,1984;Cavanaghetal.,1986a).Incontrast,mostoftheSglycoproteinisexposed.Scomprisestwoorthreecopiesofeachoftw
7、oglycopolypeptides,SI[mol.wt.about90000(90K)]and$2(about84K)derivedbycleavageofaprecursorglycopolypeptide(Stern&Sefton,1982;Cavanaghetal.,1986b).S1,butnot$2,MorNproteins,canberemovedbyurea(Cavanagh,1983b),whichledtotheproposalthatSwasanchoredinthemembraneby$2whileS1mig
8、htformthemajorpartofthedistal,bulbousendofS.Thisviewwassupportedbythefindingthatoftwomonoclonalantibodieswhichneutral
