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1、$24JournalofClinicalVirology2006,Vol36(suppl2)Abstracts,9thESCVAnnualMeetingIPConsequencesofmandatoryscreeningincornealeasyMAGplusoneofRocheMagnaPure,GentraorQiagenmeth-transplantationods.SamplesincludedEDTAwholeblood,plasma,serum,CSF,faeces,NPA,swabs(genital,throatandnose)andurine(n=181).
2、RE.Klapper1,S.S.Thomas1,K.J.Mutton1,A.J.Turner1,A.B.Tullo2,StabilityofEnteroviralRNAwastestedbystorageatambienttem-I.Zambrano2,RCarley2,A.Taylor2.1ClinicalVirology,Manchesterperature,+4~-20~and-70~andtestedatintervalsofzero,MedicalMicrobiologyPartnership,ManchesterRoyalInfirmary,two,fouran
3、dsevendays.2Corneal-TransplantService-ManchesterEyebank,Manchester,Results:SamplesextractedbyeasyMAG,whentestedby'real-UKtime'PCRassayshadacomparablelevelofsensitivitytoMagna-Pure,QiagenorGentramethods.Therewasnochangeinthesen-Background:HumanT-celllymphotropicvirus(HTLV)infectionsitivityo
4、ftheEnteroviralRT-PCRwhentestedusingRNAextractedisassociatedwithadultT-celllymphoma(ATL),tropical-spastic-byeasyMAGandstoredatambienttemperature,+4~-20~andparaparesis(TSP)andHTLV-associated-myelopathy(HAM).In2004-70~andoverthedifferenttimeintervals.mandatoryscreeningofcornealdonorsforHTLVw
5、asintroduced.DiscussionandConclusion:EasyMAGprocessesdifferentWeauditedHTLVscreeningofcadavericcornealdonorsattheCTS-sampletypesofdifferentinputandelutionvolumeswithDNAandManchesterEyeBank.RNAextractedconcurrently.DNAandRNAqualitywascomparablePatientsandMethods:Aretrospectiveanalysisoflabo
6、ratorywithautomatedandmanualextractionmethods.AdisadvantageofandtransplantationrecordsofcornealdonationsfromAugust2004easyMAGisthatsamplescannotbeprocessedovernight.EasyMAGtoDecember2005wascarriedout.ResultsofHTLVinitialreactivityextractedRNAhadexcellentstabilitywithnoevidenceofdegrada-and
7、subsequentconfirmationoftestreactivitywascomparedwithtionwhenstoredatambienttemperatureforsevendays.EyeBankdataoncornealtissueuse.Results:1009cornealdonorsweretested.73(7.2%)wereinitiallyreactive.EthnicoriginofallinitiallyreactivesampleswasCau-1~Improvedlabora
