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1、VeterinaryMicrobiology,28(1991)13-2413ElsevierSciencePublishersB.V.,AmsterdamAntigenicanalysisoffelinecoronaviruseswithmonoclonalantibodies(MAbs)"PreparationofMAbswhichdiscriminatebetweenFIPVstrain79-1146andFECVstrain79-1683T.Hohdatsua,T.Sasamotob,S.OkadabandH.KoyamabaDepartmentofVeterina
2、ryMicrobiology,bVeterinaryInfectiousDiseases,SchoolofVeterinaryMedicineandAnimalSciences,KitasatoUniversity,Towada-Shi,Aomori-Ken034,Japan(Accepted5December1990)ABSTRACTHohdatsu,T.,Sasamoto,T.,Okada,S.andKoyama,H.,1991.Antigenicanalysisoffelinecoronavi-ruseswithmonoclonalantibodies(MAbs):
3、PreparationofMAbswhichdiscriminatebetweenFIPVstrain79-1146andFECVstrain79-1683.Vet.Microbiol.,28:13-24.Weprepared31monoclonalantibodies(MAbs)againsteitherFIPVstrain79-1146orFECVstrain79-1683,andtestedthemforreactivitywithvariouscoronavirusesbyindirectfluorescentantibodyassay(IFA).SixteenM
4、Abswhichreactedwithallofthe11strainsoffelinecoronaviruses,alsoreactedwithcaninecoronavirus(CCV)andtransmissiblegastroenteritisvirus(TGEV).Inmanyofthem,thepolypeptidespecificitywastherecognitionoftransmembrane(El)proteinofthevirus.Wesuc-ceededinobtainingMAbswhichdidnotreactwitheightstrains
5、ofFIPVTypeIviruses(showingcell-associatedgrowth)butreactedwithFIPVTypeII(79-1146,KU-1)and/orFECVTypeII(79-1683)viruses(showingnon-cellassociatedgrowth).TheseMAbsalsoreactedwithCCVorTGEV.TheseMAbsrecognizedpeplomer(E2)glycoprotein,andmanyantigenicdifferenceswerefoundinthisE2protein.Thesere
6、sultssuggestthatFIPVTypeIIandFECVTypeIIvirusesareantigenicallyclosertoTGEVorCCVthantoFIPVTypeIviruses.Furthermore,theMAbpreparedinthisstudyhasenableddis-criminationbetweenFIPVstrain79-1146andFECVstrain79-1683,whichwasthoughttobeimpos-siblebythepreviousserologicalmethod.INTRODUCTIONForsero
7、logicaldiagnosisoffelineinfectiousperitonitisvirus(FIPV)infec-tion,detectionofantibodybyindirectfluorescentantibodyassay(IFA)ispopular(Pedersen,1976b;HorzinekandOsterhaus,1979;Scott,1979).Ontheotherhand,felineentericcoronavirus(FECV)whichantigenicallycross-reactswit
