冠状病毒Coronavirusl论文-1993 [Advances in Experimental Medicine and Biology] Coronaviruses Volume 342 __ Further Characterization of Aminopeptid.pdf

《冠状病毒Coronavirusl论文-1993 [Advances in Experimental Medicine and Biology] Coronaviruses Volume 342 __ Further Characterization of Aminopeptid.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、FURTHERCHARACTERIZATIONOFAMINOPEPTIDASE-NASARECEPTORFORCORONAVIRUSESBernardDelmas,1JacquelineGelfi,1HansSjostrom,2OveNoren,2andHubertLaudelIUnitedeVirologieetImmunologieMoleculairesInstitutNationaldelaRechercheAgronomique78352Jouy-en-Josas,France2DepartmentofB

2、iochemistryC,PanumInstituteUniversityofCopenhagen,DK2200DenmarkABSTRACTWerecentlyreportedthatporcineaminopeptidase-N(pAPN)actsasareceptorfortransmissiblegastroenteritisvirus(TGEV).Inthepresentwork,weaddressedthequestionofwhetherTGEVtropismisdeterminedonlybythe

3、virus-receptorinteraction.Tothisend,differentnon-permissivecelllinesweretransfectedwiththeporcineAPNeDNAandtestedfortheirsusceptibilitytoTGEVinfection.ThefourtransfectedcelllinesshowntoexpresspAPNattheirmembranebecamesensitivetoinfection.Twoofthesecelllineswer

4、efoundtobedefectivefortheproductionofviralparticles.Thissuggeststhatotherfactor(s)thanpAPNexpressionmaybeinvolvedintheproductionofinfectiousvirions.ThepAPN-transfectedcellswerealsotestedfortheirsusceptibilitytoseveralviruseswhichhaveacloseantigenicrelationship

5、toTGEV.Sofar,wefailedtoevidencepermissivitytothefelineinfectiousperitonitiscoronavirusFIPVandcaninecoronavirusCCV.Incontrast,wefoundclearevidencethatporcinerespiratorycoronavirusPRCV,avariantofTGEVwhichreplicatesefficientlyintherespiratorytractbuttoaverylowext

6、entinthegut,mayalsoutiliseAPNtogainentryintothehostcells.ThissuggeststhattheswitchbetweenTGEVandPRCVtropismsinvivomayinvolveotherdeterminant(s)thanreceptorrecognition.INTRODUCTIONCoronavirusesarecharacterizedbyarestrictedhostrangeandtissuetropismI.Thus,TGEVrep

7、licatesselectivelyintheenterocytescoveringthevilliofthesmallintestine2,whereashumancoronavirus229E(HCV-229E)multipliesintheupperrespiratorytract3.ItwasrecentlyreportedthatAPN/CD13actsasareceptorforthesetwovilUseS4.5.APN/CD13isexpressedinalargevarietyoftissuesa

8、ndcells-notablyepithelialandmyeloidcells-(6,7andreferencestherein).ThehighestAPNactivityisassociatedwiththebrushbordermembraneoftheenterocytesandoftherenalproximaltubulecells.Thedi