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1、520BriefcommunicationsJVetDiagnInvest14:520±522(2002)DetectionoffelinecoronavirusincaptiveFelidaeintheUSAMelissaKennedy,ScottCitino,AmandaHillisMcNabb,AmySerinoMoffatt,KarenGertz,StephenKaniaAbstract.Felinecoronavirus(FCoV)isanimportantpathogenofdomesticandnondomesticFelidae
2、.In-vestigationintotheprevalenceofFCoVinexoticFelidaehasreliedprimarilyonserology.TheusefulnessofgeneticdetectionofFCoVusingreversetranscriptionandnestedpolymerasechainreaction(RT/nPCR)forviralscreeningwasinvestigated.Seventy-®vebiologicsamples,primarilyfeces,fromcaptivefeli
3、dsfrom11institutionsweretestedusingPCR.Serumsamplescollectedfromallbut12oftheseanimalsweretestedforantibodiestotypeIandtypeIIFCoVbyindirectimmuno¯uorescence.Twenty-fouranimalswerepositiveusingRT/nPCRforvirus.Twenty-nineanimalswereseropositivetotypeIand/ortypeIIFCoV.Fromserol
4、ogicdata,infectionwithavirusantigenicallyrelatedtoFCoVtypeIoccurredmostcommonly.Serologydidnotcorrelatewithvirussheddingbecause13animalswereseronegativetoFCoVtypeIandIIbutpositiveusingRT/nPCRforvirus.Conversely,20animalswereseropositivebutnegativeusingRT/nPCRforFCoV.Someofth
5、epop-ulationsinwhichviruswasdetectedhadexperiencedhealthproblems,includingfelineinfectiousperitonitis(FIP),necrotizingcolitis,andmildenteritis.InadditiontoitsroleinFIP,thisvirusmayplayaroleingastro-intestinaldiseasesofinfectedanimals.ThisstudydemonstratesthatFCoVisasigni®can
6、tinfectiousagentofcaptivefelidsbecauseoverhalfoftheanimalstestedwerepositivebyviralgeneticdetection,serology,orboth.Dependenceupononemethodfordetectionofinfectionisunreliable.Infectiousdiseasesarepotentiallydevastatingtowildlifefrom11zoologicinstitutionsintheUSAforFCoVgeneti
7、cconservation.Inparticular,largecarnivorepopulationsaredetection.SpeciesofFelidaetestedincludedcheetah,snowsusceptibletoseriousconsequencesbecausetheyarealreadyleopard,Africanleopard,serval,Bengaltiger,Siberiantiger,threatenedbyrestrictedranges,habitatdestruction,andover-mou
8、ntainlion,Africanlion,lynx,ocelot,jaguar,andbobcat.exploitationofthemselves
