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1、JournalofGeneralVirology(2009),90,17241729DOI10.1099/vir.0.009704-0ComparisonofvesicularstomatitisvirusShortCommunicationpseudotypedwiththeSproteinsfromaporcineandahumancoronavirus111,2ChristelSchwegmann-Weßels,Jo¨rgGlende,XiaofengRen,3341XiuxiaQu,HongkuiDeng,Lui
2、sEnjuanesandGeorgHerrlerCorrespondence1InstituteofVirology,UniversityofVeterinaryMedicineHannover,Hannover,GermanyChristelSchwegmann-Weßels2CollegeofVeterinaryMedicine,NortheastAgriculturalUniversity,59MucaiStreet,christel.schwegmann@tiho-150030Harbin,PRChinahann
3、over.de3DepartmentofCellBiologyandGenetics,CollegeofLifeSciences,PekingUniversity,Beijing100871,PRChina4CentroNacionaldeBiotecnologı´a,CSIC,DepartmentofMolecularandCellBiology,CampusUniversitariodeCantoblanco,Darwin3,28049Madrid,SpainThesurfaceproteinsSofsevereac
4、uterespiratorysyndromecoronavirus(SARS-CoV)andtransmissiblegastroenteritisvirus(TGEV)werecomparedfortheirabilitytomediateinfectionofviralpseudotypesbasedonvesicularstomatitisvirus(VSV).Thecelltropismoftherespectivepseudotypescorrespondedtothetropismofthevirusesfr
5、omwhichtheSproteinwasderived.HigherinfectivityvalueswereobtainedwiththeSARS-CoVSproteinthanwiththeTGEVSprotein.DifferenceswereobservedwithrespecttotheimportanceofthecytoplasmictailandthemembraneanchoroftheSproteins.InthecaseoftheSARS-CoVSprotein,truncationofthecy
6、toplasmictailresultedinincreasedinfectivity.FortheTGEVSprotein,theinactivationofanintracellularretentionsignalinthecytoplasmictailwasrequired.ExchangeofthemembraneReceived16December2008anchoroftheSproteinsledtoalowinfectionefficiency.Ourresultsindicatethatrelated
7、Accepted3March2009glycoproteinsmayshowsubstantialdifferencesintheirabilitytomediatepseudotypeinfection.Surfaceproteinsofenvelopedvirusesplayanimportantluciferasetodemonstrateinfectionoftargetcells(Chanroleintheinfectionoftargetcells.Theymediatetheetal.,2000).Thel
8、atterenzymemakestheassayveryattachmentofvirionstothecellsurfaceandthesubsequentsensitive,althoughitdoesnotindicatethenumberoffusionoftheviralandthecellularmemb
