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1、InhibitionofnovelbcoronavirusreplicationbyacombinationofSUBJECTAREAS:ANTIVIRALSinterferon-a2bandribavirinSARSVIRUS11112DarrylFalzarano,EmmiedeWit,CynthiaMartellaro,JulieCallison,VincentJ.MunsterANTIVIRALAGENTS&HeinzFeldmann1,3VIRALINFECTION12DiseaseModelingandTransmissionUnit,VirusEcologyUni
2、t,LaboratoryofVirology,DivisionofIntramuralResearch,NationalReceivedInstituteofAllergyandInfectiousDiseases,NationalInstitutesofHealth,RockyMountainLaboratories,Hamilton,MT,USA,6March20133DepartmentofMedicalMicrobiology,UniversityofManitoba,Winnipeg,MB,Canada.Accepted27March2013Theidentifica
3、tionofanovelbcoronavirus,nCoV,asthecausativeagentofsevererespiratoryillnessinhumansoriginatinginSaudiArabia,QatarandJordanhasraisedconcernsaboutthepossibilityofaPublishedcoronaviruspandemicsimilartothatofSARS-CoV.Asadefinitivetreatmentregimenhasneverbeen18April2013thoroughlyevaluatedforcoron
4、avirusinfections,thereisanurgentneedtorapidlyidentifypotentialtherapeuticstoaddressfuturecasesofnCoV.Todetermineaninterventionstrategy,theeffectofinterferon-a2bandribavirinonnCoVisolatehCoV-EMC/2012replicationinVeroandLLC-MK2cellswasCorrespondenceandevaluated.hCoV-EMC/2012wassensitivetobothi
5、nterferon-a2bandribavirinaloneinVeroandLLC-MK2cells,butonlyatrelativelyhighconcentrations;however,whencombined,lowerconcentrationsofrequestsformaterialsinterferon-a2bandribavirinachievedcomparableendpoints.Thus,acombinationofinterferon-a2bandshouldbeaddressedtoribavirin,whicharealreadycommon
6、lyusedintheclinic,maybeusefulforpatientmanagementintheeventH.F.(feldmannh@offuturenCoVinfections.niaid.nih.gov)novelbcoronavirus(nCoV),hasbeenidentifiedastheetiologicalagentof17confirmedcases(11deaths)12ofasevererespiratoryillnesswithoccasionalrenalfailurefrompatientsinSaudiArabia,Qatar,Jord
7、anA3,41,5andtheUnitedKingdom.Completegenomesequencingdeterminedthatthisnewvirusisclosely6relatedtotwoAsianbatbetacoronaviruses(HKU4andHKU5)inlineageC.ThismakesnCoVthefirstlineageC5betacoronavirusknowntoinfecthumans.Whilehuman-to-humantransmissionis
