化脓性脑膜炎血液中性粒细胞CD64的测定及临床意义.doc

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1、化脓性脑膜炎血液屮性粒细胞CD64的测泄及临床意义［摘要］目的：探讨屮性粒细胞CD64、肿瘤坏死因了-a(TNF-a)水平在化脓性脑膜炎屮的诊断价值。方法：选取化脓性脑膜炎患者30例及病毒性脑炎患者36例为研究对彖，非屮枢神经系统疾病患者32例作为对照组，采用流式细胞术测定乞组血液屮性粒细胞CD64的表达，采用双抗体夹心酶联免疫吸附法测定各组血液'I«TNF-

2、分别与病毒性脑炎组和对照纟fl比较差异有显著性(PC0.01)。CD64诊断化脓性脑膜炎的敏感度为87%,特异度为貂%；TXF-a诊断化脓性脑膜炎的敏感度为66%,特异度为62%。结论：化脓性脑膜炎患者外周血屮性粒细胞CD64的表达明显增高，提示屮性粒细胞CD64可以作为诊断化脓性脑膜炎的可靠的实验室指标。［关键词］脑膜炎；CD64；肿瘤坏死因了［中图分类号］R512.3［文献标识码］A［文章编号11673-7210(2009)03(a)-019-03Detectionandclinicalsignificaneeofpuru

3、lentmeningitisbloodneutrophilCD64YANHai-qing(TheSecondSectionofDepartmentofNeurology,theFirstAffiliatedHospitalofXinxiangMedicalCollege,Weihui453100,China)［Abstract］Objective:ToexplorethediagnosticvalueofneutrophilCD64andtumornecrosisfactora(TNF-a)levelsinpurulentme

4、ningitis.Methods:30patientswithpurulentineningitisand36patientswithviralencephalitiswereselectedassubjects,and32patientswithnon-centralnervoussystemdiseasesservedascontrol,thenflowcytometrywasusedtodetecttheexpressionofneutrophilofCD64ineachgroup,anddouble-antihodys

5、andwichenzyme-linkedimmunosorbentassaywasusedtodetermineTNF-acontentineachgroup.ResuIts:ThegroupneutrophilCD64expressionlevelofpurulentmeningitiswassignificantlyhigher,andshowedsignificant.differeneerespectivelycomparedwiththatofviralcncephalitisgroupandcontrolgroup

6、(P<0.01),thelevelofTNF-ciofpurulentmcningitisgroupwassignificantlyhigher,andshowedsignificantdifferencerespectivelycomparedwiththatofviralencephalitisgroupandcontrolgroup(P<0.01).ThesensitivityCDof64indiagnosingpurulentmeningitiswas87%,andthespecificitywas88%.Thesen

7、sitivityofTNF-aindiagnosingpurulentmeningitiswas66%,andthespecificitywas62%・Conclusion:ThencutrophilCD64expressionofpatientswithpurulentmeningitiswassignificantlyincreased,suggestingthatneutrophilCD64coulclserveasareliablelaboratoryindicatorsofdiagnosingdiagnosisofp

8、urulentmeningitis.［Keywords］Meningitis;CD64;Tumornecrosisfactor化脓性脑膜炎是常见的屮枢神经系统感染性疾病，具有很高的病死率和后遗症发生率，是否及时治疗是影响其预后的重要因素Z—［1,2］。日前对化脓性脑膜炎的诊断