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《鸡肾型传染性支气管炎的初步诊断与治疗.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、南方农业学报2011。42(2):213—216JournalofSouthernA鲥culture·213·鸡肾型传染性支气管炎的初步诊断与治疗李瑞明1,史玉静z,韩涛·,韩忠燕1,宋小白p(1广西大学动物科学技术学院,南宁530005;2扬州大学兽医学院,江苏扬州225009)摘要:【目的】研究河北秦皂岛地区的鸡肾型鸡传染性支气管炎(IB)流行病学、分离鉴定地方代表毒株,为有效防制当地鸡肾型IB及研制具有免疫针对性的疫苗制剂提供参考依据。【方法】通过流行病学调查、临床症状、病理变化观察,取新鲜病死雏
2、鸡肝脏、肾脏组织悬浮液接种10日龄SPF鸡胚尿囊腔,收集鸡胚尿囊液,分别进行琼脂扩散试验、血细胞凝集(HA)试验和动物试验,并对症进行综合治疗。【结果】接种鸡胚出现典型的蜷缩、侏儒等病变,尿囊液琼脂扩散试验结果表现为阳性,经血清学试验及动物试验证实,该分离毒株为鸡传染性支气管炎病毒(IBV),结合病理变化,确诊为鸡肾型IB。经对症治疗5d后,病鸡死亡率由1.0%下降至0.4%,鸡群呼吸道症状减轻,产蛋量同升,治愈率为92.4%。【结论】该起病例为鸡。肾型IB,防制该病时应根据毒株性状针对性地选择用药及免
3、疫接种。关键词:鸡肾型传染性支气管炎;鉴定;诊断;治疗中图分类号:$858.31文献标识码:A文章编号:2095—1191(2011)02—0213-04PrimarydiagnosisandtherapytestsofnephropathogenicavianinfectiousbronchitisLIRui—ming1,SHIYu-jin92,HANTa01,HANZhong-yanl,SONGXiao-bail’(1CoHegeofAnimalScienceandTechnology,Guangx
4、iUniversity,Nanning530005,China;2VetefinaryCollege,YangzhouUniversity,Yangzhou,Jiangsu225009,China)Abstract:【Objective】Thepresentstudyw鹊conductedtoinvestigatetheepidemiologyofavianinfectiousbronchitis(IS)inQinhuangdaocityofHebeiprovince,andtoisolatethelo
5、calrepresentativestrainofIBvirusfordevelopmentofimmuo-vaccine.【Method]Theepidemiologyinvestigation,clinicalmanifestationsandpathologicalchangesinIBwererecord-ed.TheIiverandkidneysuspensionoffreshlysampledchickenwereinoculatedtoten—davSPFchickembryoallant
6、oiccavityandthechickenembryoallantoissolutionwascollectedtoconductagardiffuse,haemagglutination(HA)andanimaltest.Thecomprehensivetherapywasalsopracticed.【Result]Thekidneyofinoculatedchickshowedswellingandwasfoundpalecolored.Whiteuratedepositioninureterwa
7、sobserved.TheagardiffusetestresultofaUantoisliquidshowedpositivereSults.鹊wellastheHAandanimaltestresultsrevealedthattheisolatedstrainwasIBvirus.Basedonpathologicaldi-agnosis.theIBwasidentifiedaftnephropathgenieIB.Theinactivatedvaccinewasmadewithisolateds
8、trainsofnephropathogenicinfectiousbronchitisviruses.After5daysoftreatmentwithHukang,Shenzhishuang,Hushentongandothermedicines.themortalityofdiseasedchickenWasrecordedfrom0.4to1.O%,thesymptominrespiratorytractofchickenpopul
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