一种基于感光干膜-铟锡氧化物电极的简易细胞阻抗传感器实现细胞形态学和阻抗信息同时检测.pdf

《一种基于感光干膜-铟锡氧化物电极的简易细胞阻抗传感器实现细胞形态学和阻抗信息同时检测.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、第28卷第6期传感技术学报Vol_28No．62015年6月CHINESEJOURNALOFSENSORSANDACTUATORSJune2015ASimpleCellularImpedanceBiosensorBasedonDryFilmPhotoresist-IndiumTinOxideElectrodeforDetectingCellularMorphologyandImpedanceInformationSimultaneouslyL／Yuan，HUFan，LIAOJuan，HULiyi，LIUBeizhong(Ce

2、ntralLaboratoryofYongchuanHospital，ChongqingMedicalUniversity，Chongqing402160，China)Abstract：ThisstudydescribesthefabricationofasimplecellularimpedancebiosensorbasedondryfilmPhotore—sist—indiumtinoxide(DFP—ITO)electrodefordetectingthecellularmorphologyandimpedancei

3、nformationsimuha—neously．Adryfilmphotoresistlayerof35micronsthicknesswaslaminatedonthesurfaceoftheITOconductiveglassasaninsulatinglayer，andthencircularholeswithdifferentdiameterswereetchedontheinsulationlayerbyphotolithographytechnique．TheexposedITOelectrode，asawor

4、kingelectrode，wasconnectedtoanAg／AgC1refer—enceelectrodeandaPtcounterelectrodethroughaclampandameasuringpooltoconstituteaThree．．electrodeim．．pedancemeasurementsystem．TheimpedanceoftheDFP—ITOelectrodeswasinvestigatedbyimpedancespectrosco-PYtechnology．Thebiocompatibi

5、lityofthedryfilmphotoresistwasinvestigatedbyexperimentsofcelladhesionandcelltoxicity．ThebiologicalbehaviorinformationaboutcelladhesionandpI‘oliferationoftheA549lungcancerontheDFP—ITOelectrodewasanalyzedbyopticalmicroscopeandimpedancespectroscopytechnologyrespective

6、ly．Re—suhsshowedthattheDFP-ITOelectrodeswithdifferentdiametershadsimilarimpedancecharacteristics．FullycureddryfilmphotoresistcouldprovideanappropriatesurfaceforA549celladhesionandhadnoobviouscytotoxicity．TilecellularimpedancesensorbasedontheDFP—ITOelectrodecouldbeu

7、sedtoobtainthemorphologicaldataandanalyzethechangesofthecytoplasmmembranecapacitance，cell-cellgapresistanceandcell—ITOelectrodegapre—sistanceduringtheprocessofadhesionandproliferationofA549cellbyopticalmicroscopeandimpedancespectrumtechnologyrespectively．Thecellula

8、rimpedancebiosensorbasedonDFP—ITOelectrodehasasimplestruc-tureandcanrealizetheDual—channelacquisitionofcellmorphologyandimpedanceinformation．Itca