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《nacl胁迫对嫁接黄瓜膜脂过氧化_省略_渗透调节物质含量及光合特性的影响_杨立飞》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、西北植物学报,2006,26(6):1195—1200ActaBot.Boreal.-Occident.Sin.文章编号:1000-4025(2006)06-1195-06NaCl胁迫对嫁接黄瓜膜脂过氧化、渗透调节X物质含量及光合特性的影响*杨立飞,朱月林,胡春梅,刘正鲁,张古文(南京农业大学园艺学院,南京210095)摘要:以日本引进的耐盐品种‘帝王新土佐’南瓜为砧木,以‘津研4号’黄瓜品种为接穗,研究了NaCl胁迫对黄瓜嫁接植株和自根植株的膜脂过氧化、渗透调节物质含量及光合特性的影响。结果表明:在NaCl胁迫下,黄瓜嫁接植株叶片超氧化物歧化酶(SOD)、过氧化物酶(POD
2、)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性均显著高于自根植株,O-·2产生速率及丙二醛(MDA)含量显著低于自根植株,嫁接植株膜脂过氧化轻于自根植株;嫁接植株叶片游离脯氨酸、可溶性糖、可溶性蛋白质等渗透物质含量均显著高于自根植株;嫁接植株的净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)、叶绿素含量均显著高于自根植株;虽然NaCl胁迫抑制光合作用,但嫁接植株仍表现出优势。以上结果证明,嫁接植株耐盐性优于自根植株。关键词:嫁接黄瓜;NaCl胁迫;膜脂过氧化;渗透调节物质;光合特性中图分类号:Q945.78文献标识码:AEffectsofNaClStr
3、essontheContentsoftheSubstancesRegulatingMembraneLipidOxidationandOsmosisandPhotosyntheticCharacteristicsofGraftedCucumber*YANGLi-fei,ZHUYue-lin,HUChun-mei,LIUZheng-lu,ZHANGGu-wen(CollegeofHorticulture,NanjingAgriculturalUniversity,Nanjing210095,China)Abstract:TheeffectsofNaClstressonthecon
4、tentsofthesubstancesregulatingmembranelipidoxidationandosmosisandphotosyntheticcharacteristicsofgraftedandscion-rootplantsandscion-rootplantsofcucumberwerestudiedwithKingshintosa,asalt-tolerantvarietyintroducedfromJapan,astherootstockandJinyan4,apompkinvariety,asthescion.Itwasshownthatunder
5、NaClstresstheSOD,POD,CATandAPXactivitiesintheleavesofthegraftedplantsweresignificantlyhigherthanthoseintheleavesofscion--·rootplants,theO2producingrateandMDAcontentintheleavesofthegraftedplantsweresignificantlylowerthanthoseintheleavesofthescion-rootplantsandthemembranelipidoxidationinthegr
6、aftedplantswasweakerthanthatinthescion-rootplants;thecontentsofsuchosmoticsubstancessuchasfreeproline,solublesugar,solubleproteinintheleavesofthegraftedplantsweresignificantlyhigherthanthoseintheleavesofthescion-rootplantsandthenetphotosyntheticrate(Pn),stomataconductanceX收稿日期:2005-12-23;修改
7、稿收到日期:2006-04-30基金项目:江苏省科技厅资助项目(BE2002304,BC2003306);教育部留学回国基金资助项目[(2001)498]作者简介:杨立飞(1980-),博士研究生。*通讯联系人。Correspondenceto:ZHUYue-lin.E-mail:ylzhu@njau.edu.cn1196西北植物学报26卷(Gs),intercellularcarbondioxideconcentration(Ci)andchlorophyllcontentofthegraftedpl