大鼠电点燃岛叶致痫模型建立后神经肽Ghrelin的表达-论文.pdf

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1、·674·宁夏医学杂志2014年8月第36卷第8期NingxiaMedJ，Aug．2014Vol36,No．Doi：10．13621／j．1001—5949．2014．08．0674·论著·入—鼠电点燃岛叶致痫模型建立后神经肽Ghrelin的表达刘诤，王峰，宋子木，刘阳，高攀，李扬，孙涛[摘要]目的电点燃大鼠岛叶建立致痫模型，检测致痫大鼠神经肽Ghrelin变化特征，并探讨其意义。方法将雄性SD大鼠用数字表法随机分为空白对照组、假手术组和点燃组，又将点燃组分为点燃后1、3、7d和10d4个亚组。立体定向下将电极植人大鼠岛叶

2、，建立致痫模型，观察大鼠行为学，监测大鼠脑电情况；Nissl染色观察海马区神经元变化情况，用放射免疫法(RIA)和免疫荧光法分别检测血清和下丘脑中Ghrelin的表达水平。结果点燃后各亚组血清Ghrelin表达水平均降低，差异有统计学意义(P<0．05)；下丘脑Ghrelin表达也明显减少，差异有统计学意义(P<0．05)；各时间亚组间比较差异无统计学意义(P>0．05)。结论电刺激岛叶可建立稳定的点燃模型，癫痫发作后Ghrelin水平明显降低，血清Ghrelin水平可以反映下丘脑中相应神经肽的变化趋势。[关键词]Ghre

3、lin；癫痫；岛叶；下丘脑；血清[中图分类号]R473[文献标识码]AExpressionofneuropeptidesghrelinininsularelectricalkindledratsmodelLIUZheng，WANGFeng，SONGZimu，Yang，GAOPan，LIYang，SUNTao．DepartmentofNeurosurgery，GeneralHospitalNingxiaMed&alUniversity，Yinchuan750004，ChinaCorrespondingauthor：SUNTa

4、o，Email：suntao@nxmu．edu．ell[Abstract]0bjectiveTosetuptheinsularelectricalkindledratsmodel，andtoobserveexpressionlevelofneuropeptidesghre—linandtoexploreitssignificance．MethodsThemaleSDratswererandomlydividedintotheblankgroup，thesham—operatedgroupandkindledgroup．Th

5、elatterweredividedto4subgroupsofld，3d，7dand10daftertheratshadbeenseizure．Bystereotacticoperation，electrodeswereplacedintheratsinsularandsetupthemode1．Changesofbehaviors，ECoGandnervecellthroughNisslstainingoftheratswereobservedsimultaneously．RIA(RadioimmunologyAssa

6、y)andImmunofluorescencewereusedtodetecttheexpressionsofGhre一1inintheserumandhypothalamusoftheepilepsyrats．ResultsFromthebehavior．theECoGandthepathologyinepilepticratscon—firmedthattheratsofelectricalkindledtoinsularcanbesuccessfullyestablishedexperimentalanimalmod

7、els．Comparedtotheblankgroup，thesham—operatedgroup，allkindledgroupsoftheseizureratshadadecreasedexpressionofghrelinthroughRIAdetectintheratsserumandImmunofluorescencedetectintheratshypothalamus．Therewasnostatisticaldifferenceamonganykindledgroups(P>0．05)．Conclusion

8、Theinsularhastheseizurepotency．AnaccuratedetectionofseHlmexpressionlevelofghrlinmayreflectthefluctu—ationtrendofneuropeptidesinhypothalamus．[Keywords]Gh