NIBP和NF-κB p65在胃癌中的表达及其临床意义-论文.pdf

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1、广西医学2014年5月第36卷第5期585NIBP和NF．KBp65在胃癌中的表达及其临床意义▲周巧黄杰安刘宝玉彭鹏刘诗权覃蒙斌(广西医科大学第一附属医院消化内科，南宁市530021，E—mail：2546235539@qq．com)【摘要】目的探讨NIBP和NF—KB065在胃癌中的表达及其临床意义。方法采用实时荧光定量PCR法、Westernblot法和免疫组化sP法，检测55例胃癌、癌旁及正常胃组织中NIBP和NF．KBp65蛋白及mRNA的表达。结果胃癌组织中NIBP和NF．KBp65mRNA和蛋白阳性表达率均高于癌旁及正常组织(P<0．05)；胃癌组织中NIBP和N

2、F-KBp65的mRNA及蛋白相对表达水平均高于癌旁及正常组织(P<0．05)。胃癌组织中NIBP与NF—KBp65呈正相关(P<0．05)。胃癌组织中NIBP和NF．KB065阳性表达率与肿瘤浸润程度、分化程度、有无远处转移及临床分期有关(P<0．05)。结论NIBP和NF．KB065在胃癌组织中高表达，可能参与了胃癌的发生和发展，且NIBP激活NF-KB065信号通路途径可能在胃癌的浸润和转移过程中起重要作用。【关键词】胃癌；NIBP；核转录因子065【中图分类号】R735．2【文献标识码】A【文章编号】0253．4304(2014)05—0585．04DoI：10．11

3、675／j．issn．0253—4304．2014．05．11ExpressionsofNIBPandNuclearFactor一p65andTheirClinicalSignificanceinGastricCarcinomaZHOUQiao，HUANG胁-An，LIUBoo-yu，PENGPeng，LIUShi·quan，QtNMeng—bin(DepartmentofGastroenterology，theFirstAffiliatedHospitalofGuangxiMedwalUniversity，Nanning530021，China)【Abstract】Obje

4、ctiveToinvestigatetheexpressionofNIK—andIKK2一bindingprotein(NIBP)andnuclearfactor—KB(NF—KB)065ingastriccarcinomaandtheirclinicalsignificance．MethodsReal—timeQT-PCR，immunohistochemistryandWesternblotwereusedtodetecttheexpressionsofmRNAandproteinofNIBPandNF-KB065in55casesofgastriccarcinomatis

5、sues，adjacenttissuesandnormaltissues．ResultsThepositiveratesofmRNAandproteinofNIBPandNF．KBwerehigheringastriccarcinomatissuesthanthoseinadjacenttissuesandnormaltissues(P<0．05、．TherelativeexpressionlevelsofmRNAandproteinofNIBPandNF-KBp65ingastriccarcinomatissuesweresignificantlyhigherthantho

6、seinadjacenttissuesandnormaltissues(P<0．05)．TherewasapositivecorrelationbetweenNIBPandNF—KB065ingastriccarcinomatissues．ThepositiveexpressionofNIBPandNF—KB065ingastriccarcinomatissueswasrelatedwithdepthofinvasion，diferentiation，distantmetastasisandclinicalstage(P<0．05)．ConclusionNIBPandNF—K

7、B065areoverexpressioningastriccarcinomatissues，theymightinvolveinthepathogenesisandprogressionofgastriccarcinomar，andtheNF-KB065signalpathwayactivatedbyNIBPmightplayanimportantroleintheprocessofinvasionandmetastasisofgastriccarcinoma．【Keywords】Gastriccar