即时浸酸显著提高滞育性家蚕卵辅酶Ⅰ和Ⅱ含量.pdf

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1、昆虫学报ActaEntomologicaSinica，January2012，55(1)：24—28ISSN0454-6296即时浸酸显著提高滞育性家蚕卵辅酶I和Ⅱ含量姚金美，万华星，司马杨虎，赵林川(苏州大学基础医学与生物科学学院，江苏苏州215123)摘要：即时浸酸在阻止家蚕Bombyxmori卵滞育发动的同时，提高了其呼吸耗氧量，抑制了山梨醇积累。本研究利用HPLC法测定了家蚕滞育卵和5min即时浸酸滞育性卵中辅酶I和Ⅱ含量。结果表明：产下后24—72h，家蚕滞育卵中NAD，NADH，NADP和NADPH含量分别下降了30％，37％，50％和4％；而即时

2、浸酸滞育性卵中分别增加了77％，46％，142％和241％。不过，即时浸酸并未显著改变滞育性家蚕卵中NADIt／NAD和NADPH／NADP比值。据此推测，即时浸酸提高滞育性家蚕卵辅酶I含量与其呼吸耗氧量增加有关；即时浸酸显著提高辅酶Ⅱ含量与山梨醇积累抑制无关，而主要与生物合成加强有关。关键词：家蚕；滞育；滞育卵；即时浸酸；辅酶I；辅酶Ⅱ中图分类号：Q966文献标识码：A文章编号：0454-6296(2012)01-0024-05InstanthydrochlorideacidsoakingenhancesNAD(H)andNADP(H)levelsindia

3、pausingeggsofthesilkworm，BombyxmoriYAOJin．Mei，WANHua—Xing，SIMAYang．Hu，zHAOLin．Chuan(CollegeofPreclinicalMedicineandBiologicalScience，SoochowUniversity，Suzhou，Jiangsu215123，China)Abstract：Fordiapausingeggsofthesilkworm，Bombyxmori，diapauseinitiationispreventedwithinstanthydrochloricac

4、id(HC1)soaking，whichcancausethemarkedenhancementofoxygenconsumptionandtheinhibitionofsorbitolaccumulation．NAD(H)andNADP(H)levelsindiapauseeggsandthosesoakedwithHCIfor5minat24hafterovipositionweredeterminedusingHPLCmethods．From24hto72hafteroviposition，levelsofNAD，NADH，NADPandNADPHind

5、iapausingeggsdecreasedby30％，37％，50％and4％，respectively，whereasthoseindiapausingeggssoakedwithHC1increasedby77％，46％，142％and241％，respectively．Moreover，nosignificantefectofHC1onbothNADH／NADandNADPH／NADPratioswasobserved．ItisSOinferredthat，indiapausingeggssoakedwithHC1，themarkedincreaseo

6、fNAD(H)isinvolvedinthemarkedenhancementofoxygenconsumption，whereastheenhancementofNADP(H)isrelatedtothestrongerbiosynthesisratherthantheinhibitionofsorbitolaccumulation．Keywords：Bombyxmori；diapause；diapauseeggs；instantHC1soaking；NAD(H)；NADP(H)家蚕Bombyxmori在胚胎发育的早期发生滞育提高了滞育家蚕卵丙酮酸激酶和乳酸

7、脱氢酶活性，(Yamashita，1996)。25oC下，家蚕滞育性卵这两种酶属于糖酵解途径，其活性提高表明即时浸(diapausingeggs)大约在产下后24h左右开始滞育酸阻止滞育发动时蚕卵呼吸代谢增强。发动，表现为呼吸耗氧量急剧下降、糖原大量转换辅酶INAD(H)包括还原型烟酰胺腺嘌呤二为山梨醇和胚胎细胞分裂逐渐放慢并且最终停止与核苷酸(reducednicotinamideadeninedinucleotide，细胞周期的G：阶段。即时浸酸可以阻止滞育性家NADH)和氧化型烟酰胺腺嘌呤二核苷酸蚕卵的滞育发动，同时显著提高其呼吸耗氧量，并(nicoti

8、namideadeninedinucl