肝癌前体细胞snail表达与肝癌术后预后相关性研究.pdf

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1、68肝胆外科杂志2015年2月第23卷第1期JournalofHepatobiliarySurgery，Vol，23，No．1，Feb．2015·基础研究·肝癌前体细胞snail表达与肝癌术后预后相关性研究杨知时，刘阳，夏勇，沈锋【摘要】目的本研究通过检测CD44分子和Snail蛋白在HCC中的表达，探究CD44和Snail蛋白与HCC患者临床病理因素以及术后预后的关系。方法利用免疫组化方法检测252名肝癌术后患者癌及癌旁组织CD44和Snail蛋白的表达。结果CD44阳性表达125例，见于细胞膜和细胞浆，Snail蛋白阳性表达121例见于细胞浆和细胞核。二者阳性表达均显著高于癌旁组织。

2、CD44和Snail蛋白共阳性表达89例，其1一，3一，5年生存率为49．4％，31．5％，24．7％，1一，3一，5年复发率为59．9％，76．6％，81．6％。COX多因素分析中，CD44和Snail共阳性表达是生存复发的独立危险因素。结论CD44和Snail共阳性表达与肝癌的复发和侵袭性密切相关，可作为预测肝癌患者术后预后的指标。【关键词】CD44抗原；Snail蛋白；肝细胞癌；预后评估【中图分类号】R735．7【文献标识码】A【文章编号】1006-4761(2015)01-0068-05PrognosticValueofHepatpcelluarCarcinomaprogenit

3、orcellsexpressingSnailinpatientsafterHepatectomy(YANGZhi—s，rang，XIAYong，eta1．EasternHepatobiliaryHospital，SecondMilitaryMedicalUnivertity，Shanghai200438，China)【Abstract】ObjectiveToinvestigatetheprognositicsignificanceofespressionsofCD44andSnailinpatientswithhepatocel—luarcarcinoma(HCC)．MethodsDat

4、awereretrospectivelycollectedfrom252consecutivepatientswhounderwenthepatectomyforHCCbetweenJanuary2008andJune2008attheEasternHepatobiliarySurgeryHospital(EHBH)．TheexpressionsofCD44andSnailwereanalyzedbyusingS-Pimmunohistochemicaltechnique．AccordingtotheexpressionsofCD44andSnail，bothpositiveexpres

5、sionsofCD44andSnailweredefinedaspositivegroup．Theprognosticriskfactorsfortumorrecurrenceandoverallsurvival(OS)ofthetwogroupwerecompared．ResultsThepositiveexpressionofCD44waslocatedinmembranesandcytoplasma，whilethatofthatofSnailwaslocatedincytoplasmaandnucleus．TheincidenceofCD44andSnailexpressionw

6、ere49．6％and48．0％respectively．whichwerestatisticallyhigherthantheexpressionsinpara—carcinomatissues．Therewere89patientswhoseexpressionsofCD4andSnailwerebothpositive．Thecomparisionofclinicalpathologicalcharacteristicsbetweenthepositiveandnegativegroupsshowedthatthepositivegrouphadahigeralanineamino

7、transferase(AIJT)，proportionofmulipletumors，pTMNstaging，andpositiverateforMVI．Thepost—operative1一，3一，5-yearrecurrencerates49．4％，31．5％，24．7％forpositivegroupand74．8％，56．5％，44．3％fornegetivegroup．And1一，3一，5-yearsurvivalrat