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时间:2020-05-14
《灵芝孢子粉抑制人卵巢癌细胞增殖及诱导凋亡的体外研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、165河北医药20I5年箜Z鲞箜塑!』!:!』:doi:10.3969/j.issn.1002-7386.2015.02.001·论著·灵芝孢子粉抑制人卵巢癌细胞增殖及诱导凋亡的体外研究王卫霄姚苗苗吕艳茹杜晓欣张晓静赵素芬【摘要】目的研究灵芝孢子粉对人卵巢癌OV2008细胞增殖及凋亡的影响,并探讨其作用机制。方法采用wsT.1比色法及显微镜观察法研究灵芝孢子粉对0V2008细胞生长抑制作用及细胞形态的改变;流式细胞仪检测细胞周期分布情况;Hoechst一33258染色方法评估细胞凋亡并采用westernblot方法测定凋亡相关蛋白的表达。结果WST结果表明灵芝孢子粉可
2、以抑制OV2008细胞的生长,并呈剂量和时间依赖性;流式细胞术结果提示灵芝孢子粉可使卵巢癌细胞生长阻滞于G1期。Hoechst一33258染色结果显示灵芝孢子处理细胞后可以检测出凋亡细胞。Westernblot方法检测到凋亡抑制蛋白bcl-2、bcl—xl随灵芝孢子浓度增加而下降,而促凋亡蛋白bax及P27、casep具有抑制卵巢癌细胞生长及诱导凋亡的作用,其机制是作用于细胞周期的ase一3逐渐升高。结论灵芝孢子粉可以抑制卵巢细胞生长,使细胞生长阻滞于G1期,并可以通过上调bax、P27,下调bcl一2并活化casepase一3而发挥其作用。【关键词】灵芝孢子粉;卵巢
3、肿瘤;增殖;凋亡;细胞周期【中图分类号】R737:31【文献标识码】A【文章编号】1002—7386(2015)02—0165—05ExperimentalstudyontheefectofGLSPonhumanovaHancancercelproliferationandapoptosisinvitroWANGWe~iao,YAOMiaomiao,LVYanru,eta1.DepartmentofObstetricsandGynecology,C.aochengPeople’SHospital,Hebei,Gaocheng052160,China【Abstract】
4、ObjectiveToinvestigatetheeffectofGanodermalucidumsporepowder(GLSP)oncellproliferationandapoptosisofhumanovariancellline-OV2008,andtoexploreitsactionmechanism.MethodsTheeffectsofGLSPonOV2008cellgrowthandcellmorphousweredetectedbyWST一1colorimetrictechniquearidmicroscopy;cellcyclewasanalyz
5、edflowcytometry;cellapoptosiswasdetectedbyHoechst一33258stainingmethod;theexpressionofapoptosis—relatedproteins(bcl一2bax,P27,cleavedCaspase一3)weremeasuredbyWesternblotting.ResultsTheresuhsofWST·1assaysshowedthatGLSPcouldinhibitthegrowthofOV2008cells,moreover,whichwastimeanddosedependent.
6、TheresultsbyflowcytometryshowedthatGLSPcouldblock0V2008cellgrowthatG1phase.Hoechst33258stainingresultsshowedthatGLSPcouldinducecellapoptosis.Westernblottingresultsshowedthattheexpressionlevelsofbcl一2,bcl—xlweredecreasedwiththeincreaseofconcentrationofGLSP,howeverbax,P27,Caspase一3couldin
7、hibitthegrowthofovariancancercellsandinducecellapoptosis,anditsactionmechanismwasthattheexpressionlevelsofCasbase一3thataffectedcellcycleweregraduallyincreased.ConclusionGLSPcaninhibitthegrowthofovariancancercells,blockcellgrowthatG1stage,furthermore,whichcandevelopitseffectsbyu
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