吉西他滨联合顺铂不同给药途径对人肺癌A549细胞的抑制作用.pdf

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1、1180TianjinMedJ，December2014，Vo1．42No．12吉西他滨联合顺铂不同给药途径对人肺癌A549细胞的抑制作用曹军。何阳刘洪强。王赛博赵保成郑晓辉程英升弘【摘要】目的对比吉西他滨联合顺铂动脉化疗与静脉化疗对人肺腺癌生长的抑制作用，探索最佳给药途径。方法选取裸大鼠40只，将人源肺腺癌A549细胞系经传代培养后，移植于裸大鼠以建立肺癌模型。将荷瘤鼠均分为荷瘤对照组、假手术对照组、静脉化疗组与动脉化疗组；荷瘤对照组尾静脉注入生理盐水；动脉化疗组经动脉注入吉西他滨150mgg+顺铂10mg／kg；静脉化疗组经静脉注入吉西他滨150mg／kg+Jl~$A10mg／kg；假

2、手术对照组经动脉注入生理盐水。观察干预前后4组大鼠肿瘤体积的变化，计算荷瘤鼠的抑瘤率，采用Westernblot检测Bcl一2与半胱氨酸蛋白酶(Caspase)一3蛋白在肿瘤中的表达水平。结果动脉化疗组与静脉化疗组移植瘤生长均受到不同程度的抑制，动脉化疗组抑制尤为明显。动脉化疗组、静脉化疗组的瘤体质量均低于荷瘤对照组(g：1．91±O．19、2．61~0．21vs4．58~0．46)，差异有统计学意义，动脉化疗组、静脉化疗组的抑瘤率分别为57．6％、42．4％。经静脉与动脉给药后，Bcl一2蛋白水平均下降，Caspase一3蛋白水平均上升，以动脉给药效果更明显。结论吉西他滨联合顺铂经动脉灌

3、注更能有效控制人肺癌细胞生长，经动脉化疗途径在肺癌治疗中更具优势。【关键词】吉西他滨；顺铂；动脉化疗；静脉化疗；肺腺癌【中图分类号】R734．2【文献标志码】A【DOI】10．3969／j．issn．0253—9896．2014．12．008InhibitingEfectofDiferentAdministrationRouteofGiemcitabineCombinedwithCisplatinonA549CellLinesCAOJun，HEYang，LIUHongqiang2,WANGSaibo，ZHAOBaocheng，ZHENGXiaohui，CHENGYingshengJMedi

4、calCollegeofSoochowUniversity,Suzhou215123，China；2DahuaHospital,．XuhuiDistrict，．Shanghai；3ShanghaiJiaoTongUniversityAffiliatedSixthPeople’sHosphalCorrespondingAuthorE—mail：chengyingsheng@hotmail．corn[Abstract】0bjiectiveTocomparetheinhibitingeffectonhumanlungadenocarcinomawhoweretreatedwithgem-cita

5、binecombinedwithcisplatinchemotherapythrougheitherarterialorintravenousroutetoexploretheoptimumadminis—trationroute．MethodsHumanlungadenocarcinomaderivedA549cellsweretransplantedinto40BALB／c—numicetoes—tablishlungcancermodelThemodelsweredividedinto4groups：animalsinarterialorintravenouschemotherapy

6、groupsweretreatedwithgemcitabine150mg／kgcombinedwithcisplatin10mg／kgthrougheitherarterialrouteorintravenousroute．Animalsinnegativecontrolgroupweregivennormalsalinethroughcaudalveinwhileanimalsinshamoperationgroupweretreatedwithnormalsalineviaarterialroute．Thendynamicalchangeoftumorvolumeandtumorin

7、hibitingratewereassessed，andBcl一2andCaspase3expressionswereinvestigatedusingwesternblot．Finallyinhibitingeffectwerecomparedbetweenthesetwodifferentadministrationroutes．Re5；ulItsTransplantedtumorsinarteri~alandint