竹炭对三叶草生长及土壤细菌群落的影响.pdf

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1、应用生态学报2014年8月第25卷第8期ChineseJournalofAppliedEcology，Aug．2014，25(8)：2334-2340竹炭对三叶草生长及土壤细菌群落的影响术李松昊何冬华沈秋兰徐秋芳一(浙江省森林生态系统碳循环与固碳减排重点实验室／浙江农林大学环境与资源学院，浙江临安311300)摘要研究添加不同含量(0、3％和9％)和颗粒直径(0．05、0．05～1．0和1．0—2．0mm)竹炭对三叶草生长及土壤微生物群落结构的影响．结果表明：竹炭对三叶草生长的促进作用前期较明显，添加9％竹炭处理略好于添加3％竹炭处理，而不同颗粒直径对三叶草生长影响差

2、异不显著；播种后0～120d，竹炭对三叶草生长的促进作用随着时间推移而减弱，5个月后基本消失．16SrDNAV3区片段的DGGE分析表明，添加竹炭改变了土壤细菌群落结构特征，大部分种类土壤细菌数量和细菌群落多样性指数均高于对照．定量分析表明，添加9％、细粒(D<0．05mm)竹炭处理土壤细菌数量显著高于其他处理．同一添加含量下，细粒径竹炭对土壤细菌数量的增加效应更明显．关键词竹炭三叶草土壤细菌群落土壤细菌丰度文章编号1001—9332(2014)08—2334—07中图分类号S154．38文献标识码AEfectsofbamboocharcoalonthegrowtho

3、fTrifoliumrepensandsoilbacterialcommunitystructure．LISong—hao，HEDong—hua，SHENQiu—lan，XUQiu-fang(ZhejiangProvincialKeyLaboratoryofCarbonCyclinginForestEcosystemsandCarbonSequestration~SchoolofEnvironmentandResourceSciences，ZhejiangA&FUniversity，Lin’an311300，Zhejiang，China)．一Chin．Ap—pf．o1

4、．，2014，25(8)：2334—2340．Abstract：Theeffectsofadditionrates(0，3％and9％)andparticlesizes(0．05．0．05—1．0and1．0—2．0mm)ofbamboocharcoalonthegrowthofTrifoliumrepensandsoilmicrobialcommunitystructurewereinvestigated．Theresultsshowedthatbamboocharcoaladditiongreatlypromotedtheearlygrowthofrepens．w

5、iththe9％charcoaladditionratebeingslightlybetterthanthe3％charcoaladditionrate．TheeffectsofdifferentparticlesizesofbamboocharcoalOfthegrowthofrepenswerenotdifferentsignificantly．Growthpromotiondeclinedwithtimeduring120daysaftersowing．anddisappearedcompletelyafter5months．DGGEanalysisoftheb

6、acterial16SrDNAV3fragmentindicatedthatbamboocharcoalalteredthesoilbacterialcommunitystructure．TheamountandShannondiversityindexofbacteriainthebamboocharcoaladditiontreatmentsincreasedcorn—paredwithCK．Thequantitativeanalysisshowedthattheamountofbacteriainthetreatmentwithbamboocharcoaloff

7、ineparticle(D<0．05mm)atthe9％additionratewassignificantlyhigherthanintheothertreatments．Thefinebamboocharcoalhadagreateffectonsoilbacteriaamountcomparedwiththecharcoalofothersizesatthesameadditionrate．Keywords：bamboocharcoal；Trifoliumrepens；soilbacterialcommunity：soilbacterialri