常用溶液的配制方法.doc

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1、常用溶剂的配制方法1.磷酸缓冲液：0.15M，pH=7.4磷酸缓冲液：KH2PO4：2.041g+100ml水K2HPO4·3H2O：10.3g+300mL水两液混合即成400mL，0.15M，pH=7.4的磷酸缓冲液0.2mol/L不同pH的磷酸缓冲液：先配制0.2mol/L的磷酸二氢钾溶液和0.2mol/L的磷酸二氢钾溶液，然后按下表配制：pHKH2PO4（mL）K2HPO4·3H2O（mL）pHKH2PO4（mL）K2HPO4·3H2O（mL）5.793.56.56.945.055.05.892.58.07.039.061.05.990.010.07.133.067

2、.06.087.712.37.228.072.06.185.015.07.323.077.06.281.018.57.419.081.06.377.522.57.516.084.06.473.526.57.613.087.06.568.531.57.710.590.06.662.537.57.88.591.56.756.543.57.97.093.06.851.049.08.05.394.72.硼酸缓冲液0.15M，pH=8.2硼酸缓冲液：四硼酸钠溶液：2g+35mL水硼酸溶液：3.246g硼酸+350mL水两液混合即成700mL，0.15M，pH=8.2的硼酸缓冲液0.

3、2mol/L（硼酸根），不同pH的硼酸缓冲液：先配制0.2mol/L的硼酸溶液和0.05mol/L的四硼酸钠溶液，然后按下表配制：pH四硼酸钠mL硼酸mLpH四硼酸钠mL硼酸mL7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.03.甘氨酸-盐酸缓冲液：0.2mol/L0.2mol/L甘氨酸溶液（15.01g/L）pH0.2mol/L甘氨酸mL0.2mol/L盐酸mLpH0.2mol/L甘氨酸mL0.2mol/L盐酸mL2.05044.43.05011.42.45032.43.25

4、08.22.65024.23.4506.42.85016.83.6505.04.柠檬酸缓冲液：0.1mol/LC6H8O7·H2O:0.1mol/L溶液为21.01g/LNa3C6H5O7·2H2O：0.1mol/L溶液为29.41g/LpH0.1mol/L柠檬酸mL0.1mol/L柠檬酸钠mLpH0.1mol/L柠檬酸mL0.1mol/L柠檬酸钠mL3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.

5、03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.44.89.210.80.1MpH=4柠檬酸缓冲液222mLV1：柠檬酸V2：柠檬酸钠V1=131mL0.1*0.131*210.14=2.75gV2=91mL0.1*0.091*294.10=2.68g0.1MpH=2.3柠檬酸缓冲液412mLV1：柠檬酸V2：柠檬酸钠V1=400mL0.1*0.4*210.14=8.40gV2=12mL0.1*0.012*294.10=0.353g1MpH=10碳酸缓冲液211mLV1：碳酸钠V2：碳酸

6、氢钠V1=150mL1*0.15*106=15.9gV2=61mL1*0.061*84=5.12g原来1MpH=7.4磷酸缓冲液203mLV1：K2HPO4·3H2OV2：KH2PO4V1=150mL0.15*1*228.22=34.23gV2=53mL0.053*1*136.08=7.21g现在1MpH=7.4磷酸缓冲液134mLV1：K2HPO4·3H2OV2：KH2PO4V1=100mL0.1*1*228.22=22.82gV2=34mL0.034*1*136.09=4.3g0.1MpH=8磷酸缓冲液20.15mLV1：K2HPO4·3H2OV2：KH2PO4V1=

7、20mL0.1*0.02*228.22=0.46gV2=0.15mL0.1*0.15*10-3*136.08=0.002g0.5MpH=8Tris-HCl缓冲液165mLV1：TrisV2：HClV1=105mL0.5*0.105*12.1.=6.36gV2=60mL（0.5MHCl）0.2MpH=4.3柠檬酸缓冲液372mLV1：柠檬酸V2：柠檬酸钠V1=300mL0.3*0.2*210.14=12.61gV2=72mL0.2*0.072*294.10=4.24g0.2MpH=4柠檬酸缓冲液340mLV1：柠檬酸V2：柠檬酸