慢性间歇低氧对大鼠血脂和ghrelin表达的影响.pdf

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1、p华老年心脑血管病杂志2o14年7月第l6卷第7期ChinJGeriatrHeartBrainVesselDis，Jul2o14，Vol16，No．7·747·基础研究幔性间歇低氧对大鼠血脂和ghrelin表达的影响陈雷，孙晓，王实，佟浩，张曼摘要：目的观察慢性间歇低氧对大鼠血脂及血管内皮和下丘脑ghrelin表达的影响。方法将健康雄性Wistar大鼠60只，随机分为正常空气组，持续低氧组和间歇低氧组，每组2o只，第l、3个月末测大鼠血脂，C反应蛋白(CRP)，RT—PCR法测下丘脑和血管内皮ghrelin基因表达，

2、Westernblot法测血管内皮血管细胞黏附分子1(VCAM一1)、细胞间黏附分子1(ICAM一1)蛋白表达。结果与正常空气组比较，持续低氧组血脂、CRP无显著变化(P>0．05)，间歇低氧组血脂、CRP明显增加(P0．05)，间歇低氧组下丘脑和血管内皮ghrelin基因表达明显降低(0．524-0．28vs0．68士0．41，0．694

3、-0．21哪!．19±0．21，P<0．05)；与正常空气组比较，持续低氧组vCAMl、IcAM一1蛋白表达无明显变化(P>0．05)，间歇低氧组VCAM一1、ICAM一1蛋白表达明显增加(P

4、Lei，SUNXiao，WANGShi，eta1(DepartmentofCardiology，Af}iliatedCentralHospitalofShenyangMedicalCollege，Shenyang110024，LiaoningProvine，China)Abstract：ObjectiveTostudytheeffectofchronicintermittenthypoxemiaonlipidlevelandghre—linexpressioninendotheliumandhypothalamuso

5、frats．MethodsSixtyhealthymaleWistarratswererandomlydividedintonormalgroup，persistenthypoxemiagroup，andintermittenthypox—emiagroup．Theirbodyweight，serumlipidandCRPlevelweremeasured，expressionsofghrelin，VCAM一1andICAM一1proteininhypothalamusandendotheliumweredetect

6、edbyRT—PCRandWesternblot，respectively，attheendofmonths1and3．ResultsNosignificantdifferencewasfoundinbodyweight，serumlipidandCRPlevelbetweennormalgroupandpersistenthypoxemiagroup(P>0．05)．Thebodyweight，serumlipidandCRPlevelweresignificantlyhigherininter—mittenthy

7、poxemiagroupthaninnormalgroup(P<0．05)．Nosignificantdifferencewasfoundinghrelinexpressionlevelbetweennormalgroupandpersistenthypoxemiagroup(P>0．05)．Theghrelinexpressionlevelwassignificantlylowerinintermittenthypoxemiagroupthaninnormalgroup(P<0．05)．Nosignificantd

8、ifferencewasfoundinVCAM一1andICAM一1expressionlevelbetweenpersistenthypoxemiagroupandnormalgroup(P~0．05)．TheVCAM一1andICAM一1ex—pressionlevelsweresignificantlyhigherinintermitte