地高辛 试剂盒说明书1.doc

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1、仅仅研究于生命科学，不适合在诊断过程使用只能在体外使用地高辛DNA标记和检测试剂盒1和NBT/BCIP一起用于颜色检测通过酶联免疫法采用地高辛-dUTP进行随机引物DNA标记，碱性标记和检测货号：11745832910此试剂盒储存条件-15oC—-25oC此试剂盒可对10ng-3ugDNA进行12次标记反应可检测100cm2面积的杂交膜24张指导手册2009.11版1前言1.1内容表1前言..................................................................................................

2、.......................................21.1内容表..................................................................................................................................21.2试剂盒内容.............................................................................................................

3、.............32简介.........................................................................................................................................52.1产品概述..............................................................................................................................53步骤和所

4、需材料.....................................................................................................................83.1开始之前..............................................................................................................................83.2地高辛DNA标记............................

5、....................................................................................93.3标记效率的测定...............................................................................................................113.4DNA的转移和固定..........................................................................

6、...............................143.5杂交....................................................................................................................................163.6免疫检测...................................................................................................................

7、.........183.7DNA印记的洗脱和再杂交..............................................................................................204结果...............................................................................................................